AI Article Synopsis
- The interface between nucleic acids and nanomaterials has become a key area for advancements in applications like sensing, bioimaging, drug delivery, and theranostics.
- A significant challenge in these systems is accurately measuring nucleic acid concentration due to interference from nanoparticles.
- This study presents a simple and reliable method using circular dichroism (CD) spectroscopy to quantify nucleic acid concentrations in nanoparticle conjugates, successfully applying it to three different types of nucleic acids.
Article Abstract
The interface of nucleic acids and nanomaterials is among the most promising fields in recent years. Considerable efforts have been devoted to the development of novel systems based on the two components for various promising applications such as sensing, bioimaging, drug delivery, and theranostics. However, the determination of nucleic acid concentration in these systems remains as a challenge due to the interference of nanoparticles. To this end, we developed a simple, yet reliable, method to quantify the nucleic acid concentration in their nanoparticle or polymer conjugates based on circular dichroism (CD) spectroscopy. In this paper, three nucleic acids, namely, DNA sodium salt from calf thymus (NaDNA), DNA from herring sperm (hsDNA), and ribonucleic acid from torula yeast (tyRNA), were noncovalently conjugated to three nanoparticles. The concentrations of the three nucleic acids in their nanoparticle conjugates were successfully determined on the basis of CD spectra calibration curves.
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| http://dx.doi.org/10.1021/acs.analchem.7b04621 | DOI Listing |
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