Liquid-liquid phase separation between aqueous solutions containing two incompatible polymers, a polymer and a salt, or a polymer and a surfactant, has been exploited for a wide variety of biotechnology applications throughout the years. While many applications for aqueous two-phase systems fall within the realm of separation science, the ability to partition many different materials within these systems, coupled with recent advances in materials science and liquid handling, has allowed bioengineers to imagine new applications. This progress report provides an overview of the history and key properties of aqueous two-phase systems to lend context to how these materials have progressed to modern applications such as cellular micropatterning and bioprinting, high-throughput 3D tissue assembly, microscale biomolecular assay development, facilitation of cell separation and microcapsule production using microfluidic devices, and synthetic biology. Future directions and present limitations and design considerations of this adaptable and promising toolkit for biomolecule and cellular manipulation are further evaluated.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/adhm.201701036 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Screening of Solvent Systems for Countercurrent Chromatography Separation of Polar Constituents from L. Seeds.
Molecules
January 2025
College of Chemistry & Pharmacy, Northwest A&F University, 22 Xiong Road, Yangling 712100, China.
The separation of large polar constituents presents a substantial challenge in natural product research when employing column chromatography techniques, as the process is both complex and time-consuming. In this study, an acetonitrile/tetrahydrofuran/di-(2-ethylhexyl) phosphoric acid/aqueous saturated sodium chloride solvent system was developed and utilized for the countercurrent chromatography of polar constituents from L. seeds.
View Article and Find Full Text PDFArtificial cells with all-aqueous droplet-in-droplet structures for spatially separated transcription and translation.
Nat Commun
January 2025
Department of Applied Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8656, Japan.
The design of functional artificial cells involves compartmentalizing biochemical processes to mimic cellular organization. To emulate the complex chemical systems in biological cells, it is necessary to incorporate an increasing number of cellular functions into single compartments. Artificial organelles that spatially segregate reactions inside artificial cells will be beneficial in this context by rectifying biochemical pathways.
View Article and Find Full Text PDFSimultaneous extraction and purification of polysaccharides and proteins from Pleurotus ostreatus using an aqueous two-phase system.
J Food Sci
January 2025
College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, China.
Pleurotus ostreatus is a nutrient-dense edible fungus renowned for its delicate texture, appealing flavor, and numerous potential health benefits. Simultaneous extraction within the framework of food resource processing facilitates the concurrent isolation and analysis of multiple target compounds. In this study, an ethanol/salt aqueous two-phase system (ATPS) was employed to extract polysaccharides (PS) and proteins from P.
View Article and Find Full Text PDFSoluplus Stabilized Amorphous Dispersions for Enhanced Oral Absorption of Felodipine.
Curr Drug Deliv
January 2025
Department of Biopharmaceutical, Lishui University, 1 Xueyuan Road, Lishui, 323000, China.
Background: Overcoming the poor aqueous solubility of small-molecule drugs is a major challenge in developing clinical pharmaceuticals. Felodipine (FLDP), an L-type calcium calcium channel blocker, is a poorly water-soluble drug.
Objectives: The study aimed to explore the potential applications of polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol (Soluplus) stabilized amorphous dispersions for augmenting the oral delivery of poorly water-soluble drugs.
Memory Effect of Double Oxides Compared to Simple Ion Exchange for Controlled Fluoride Ion Capture and Release.
Materials (Basel)
January 2025
Faculty of Technology and Metallurgy, University of Belgrade, 11000 Belgrade, Serbia.
Article Synopsis
- A magnesium-aluminum layered double hydroxide (LDH) was created using a coprecipitation technique from a nitrate solution and transformed into a layered double oxide (LDO) after being heated to 450 °C.
- During rehydration in a fluoride solution, the LDH's original structure was restored and fluoride ions were absorbed to maintain balance, a finding confirmed by energy-dispersive X-ray spectroscopy (EDS).
- The study demonstrated that using ethanol during the rehydration process significantly increased fluoride incorporation, and the fluoride release pattern from the material revealed a rapid initial release followed by a slower, prolonged release.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!