Yeasts are promising alternative hosts for the manufacturing of recombinant protein therapeutics because they simply and efficiently meet needs for both platform and small-market drugs. Fast accumulation of biomass and low-cost media reduce the cost-of-goods when using yeast, which in turn can enable agile, small-volume manufacturing facilities. Small, tractable yeast genomes are amenable to rapid process development, facilitating strain and product quality by design. Specifically, Pichia pastoris is becoming a widely accepted yeast for biopharmaceutical manufacturing in much of the world owing to a clean secreted product and the rapidly expanding understanding of its cell biology as a host organism. We advocate for a near term partnership spanning industry and academia to promote open source, timely development of yeast hosts.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.copbio.2017.12.010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Non-conventional yeasts: promising cell factories for organic acid bioproduction.
Trends Biotechnol
January 2025
Department of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:
Microbial production of organic acids has been hindered by the poor acid tolerance of microorganisms and the high costs of waste salt reprocessing. The robustness of non-conventional microorganisms in an acidic environment makes it possible to produce organic acids at low pH and greatly simplifies downstream processing. In this review we discuss the environmental adaptability features of non-conventional yeasts, as well as the latest developments in genomic engineering strategies that have facilitated metabolic engineering of these strains.
View Article and Find Full Text PDFPrenylation-dependent membrane localization of a deubiquitinating enzyme and its role in regulating G protein-mediated signaling in yeast.
J Biol Chem
January 2025
Department of Biology, Saint Louis University, St. Louis, MO 63103. Electronic address:
Miy1 is a highly conserved de-ubiquitinating enzyme in yeast with MINDY1 as its human homolog. Miy1 is known to act on K48-linked polyubiquitin chain, but its biological function is unknown. Miy1 has a putative prenylation site, suggesting it as a membrane-associated protein that may contribute to the regulation of cell signaling.
View Article and Find Full Text PDFmTOR signalling controls protein aggregation during heat stress and cellular aging in a translation- and Hsf1-independent manner.
J Biol Chem
January 2025
Institute for Biomedicine, Sahlgrenska Academy, Centre for Ageing and Health-AgeCap, University of Gothenburg, Sweden. Electronic address:
The mTOR (mechanistic target of rapamycin) signaling pathway appears central to the aging process as genetic or pharmacological inhibition of mTOR extends lifespan in most eukaryotes tested. While the regulation of protein synthesis by mTOR has been studied in great detail, its impact on protein misfolding and aggregation during stress and aging is less explored. In this study, we identified the mTOR signaling pathway and the linked SEA complex as central nodes of protein aggregation during heat stress and cellular aging, using Saccharomyces cerevisiae as a model organism.
View Article and Find Full Text PDFThe bHLH transcription factor DlbHLH68 positively regulates DlSPS1 expression to promote sucrose biosynthesis in longan.
Int J Biol Macromol
January 2025
College of Horticulture, Institute of Genetics and Breeding in Horticultural Plants, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address:
Sucrose is an important factor affecting plant growth and fruit quality, but the molecular regulatory mechanism of sucrose biosynthesis in longan is not yet understood. Here, we characterized a transcription factor, DlbHLH68, positively regulates sucrose accumulation in longan. Subcellular localization and transcriptional activity analysis indicated that DlbHLH68 is a nuclear transcriptional activator.
View Article and Find Full Text PDFWD40 proteins PaTTG1 interact with both bHLH and MYB to regulate trichome formation and anthocyanin biosynthesis in Platanus acerifolia.
Plant Sci
January 2025
Anhui Province Key Laboratory of Forest Resources and Silviculture, School of Forestry and Landscape Architecture, AnHui Agricultural University, HeFei 230036, PR China. Electronic address:
Trichome development and anthocyanin accumulation are regulated by a complex regulatory network, the MBW complexes consist of MYB, bHLH, and WD40 transcription factors. In this study, two sequences, named PaTTG1.1, and PaTTG1.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!