AI Article Synopsis
- Researchers studied how calcium (Ca) changes during dengue virus (DENV) infection in liver cells.
- They found that DENV-infected HepG2 cells let in more Ca, while both HepG2 and Huh-7 cells had less Ca stored inside them.
- When they changed the Ca levels in the infected cells, it made the DENV replication go down, showing that Ca plays an important role in helping the virus spread.
Article Abstract
The role of Ca during dengue virus (DENV) replication is unknown; thus, changes in Ca homeostasis in DENV infected human hepatic HepG2 and Huh-7 cells were analyzed. Infected HepG2 cells, but not Huh-7 cells, showed a significant increase in plasma membrane permeability to Ca, while both cell lines showed marked reduced levels of Ca stored in the endoplasmic reticulum. While the expression levels of STIM1 and ORAI1 showed no changes, STIM1 and ORAI1 were shown to co-localized in infected cells, indicating activation of the store-operated Ca entry (SOCE) pathway. Finally, manipulation in the infected cells of the intra and extracellular Ca levels by chelators (BAPTA-AM and EGTA), SOC inhibitor (SKF96365), IP3 Receptor antagonist (2APB) or increase of extracellular [Ca], significantly reduced DENV yield, but not vesicular stomatitis virus yield, used as a control. These results show that DENV infection alters cell Ca homeostasis and that such changes favor viral replication.
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| http://dx.doi.org/10.1016/j.virusres.2017.11.029 | DOI Listing |
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