AI Article Synopsis
- The microtubule organizing center and transient microtubule array (MTOC-TMA) in Xenopus oocytes forms at the vegetal side of the nucleus through actin reorganization, but the molecular coordination between actin and microtubules during this process is not fully understood.
- Research indicates that the actin nucleation promoting factor WASH plays a crucial role in MTOC-TMA assembly, and its depletion disrupts the alignment between actin filaments and microtubules.
- Further analysis shows that the WHD2 domain of WASH negatively regulates the activity of the VCA domain, leading to abnormal actin accumulation and malformation of MTOC-TMA when WHD2 is removed, demonstrating
Article Abstract
During Xenopus oocyte maturation, the microtubule organizing center and transient microtubule array (MTOC-TMA) forms at the vegetal side of the nucleus and we have previously demonstrated that this formation requires actin reorganization. However, the molecular mechanism that coordinates actin filaments with microtubules (MTs) during oocyte maturation is unknown. Here we show that the actin nucleation promoting factor WASH is involved in MTOC-TMA assembly. WASH depletion disrupted the alignment of actin filaments and MT at the base of MTOC-TMA. In vitro cosedimentation assays revealed that the Xenopus WASH VCA domain, which comprises the actin and Arp2/3 complex binding region, directly bound to MT but that the WHD2 domain, which had been previously reported to be an MT-binding domain of mammalian WASH, did not. Injection of the WHD2-deleted mutant into oocytes caused a drastic accumulation of actin filaments in the cytoplasm and malformation of MTOC-TMA, suggesting that the WHD2 domain negatively regulates the VCA domain activity during oocyte maturation. Our results suggest that WASH complex-dependent actin nucleation is involved in the assembly of MTOC-TMA.
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