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Typing Arctic and Africa-2 clades of rabies virus using clade-defining single nucleotide polymorphisms.
Arch Microbiol
January 2025
Department of Biochemistry, Division of Biological Sciences, Indian Institute of Science, Bangalore, India.
Rabies is a deadly neurotropic, zoonotic disease with a mortality rate of 100% after symptoms appear. Rabies virus (RABV) is the primary cause of rabies disease in humans, and it mainly spreads via dog bites in developing countries. Over the course of RABV evolution, multiple RABV variants, called clades, have emerged.
View Article and Find Full Text PDFPerformance of multiplex PCR-based targeted next-generation sequencing in bronchoalveolar lavage fluid for the diagnosis of invasive pulmonary aspergillosis in non-neutropenic patients.
J Infect Dis
January 2025
Department of Pulmonary and Critical Care Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei, P.R. China.
Background And Objective: Multiplex polymerase chain reaction (PCR)-based targeted next-generation sequencing (tNGS) is a promising tool for distinguishing lower respiratory tract infections (LRTIs) in clinical practice, and its detectable pathogen spectrum can cover more than 95% of clinical cases. but there is limited information on systematic evaluation of the clinical use of multiplex PCR-based tNGS (mp-tNGS) in IPA cases. We aim to assess mp-tNGS in bronchoalveolar lavage fluid (BALF) for Aspergillus detection in suspected IPA patients, and to provide a reliable basis for initiating antifungal therapy without microbiological or histopathological evidence.
View Article and Find Full Text PDFMetagenomics: a new frontier for routine pathology testing of gastrointestinal pathogens.
Gut Pathog
January 2025
Microba Pty Ltd, Brisbane, Australia.
Background: Accurate and comprehensive identification of enteropathogens, causing infectious gastroenteritis, is essential for optimal patient treatment and effective isolation processes in health care systems. Traditional diagnostic techniques are well established and optimised in low-cost formats. However, thorough testing for a wider range of causal agents is time consuming and remains limited to a subset of pathogenic organisms.
View Article and Find Full Text PDFAssessment of typing methods, virulence genes profile and antimicrobial susceptibility for clinical isolates of Proteus mirabilis.
Ann Clin Microbiol Antimicrob
January 2025
Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
Proteus mirabilis (P. mirabilis) is one of the most important causative pathogens associated with complicated urinary tract infections with a 20% incidence. For epidemiological determinations, several phenotypic and molecular typing methods have been implicated.
View Article and Find Full Text PDFKey attributes of nitrocellulose-based energetic materials and recent developments.
Heliyon
January 2025
Centre for Tropicalization (CENTROP), Defence Research Institute, Universiti Pertahanan Nasional Malaysia, Kem Sungai Besi, 57000, Kuala Lumpur, Malaysia.
Nitrocellulose (NC)-based propellants have played a pivotal role in the development of energetic materials for both military and civilian applications. This review offers a comprehensive exploration of NC-based propellants, tracing their evolution from their historical origins as smokeless gunpowder to modern advancements. It discusses the chemical composition and classifications of NC propellants, along with continuous efforts to refine smokeless powder formulations through studies on smoke formation, residues, and additives.
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