Comparative Physicochemical Evaluation of Kharekhasak ( Linn.) Before and After Mudabbar Process.

Background And Objectives: Mudabbar/ is referred to the processes performed on the drugs to detoxify, purify, and enhance therapeutic action and to reduce its doses before making the formulations in Unani medicine. It improves quality of drugs either by optimizing its desirable characteristics or minimizing the undesirable ones; it makes drug effective, safe, and specific. There is a need of comparative evaluation to understand its significance. Tadbeer of Kharekhasak (KK) khurd ( Linn. fruit) is described by Rabban Al-Tabari in Firdausul Hikmat, Akbar Arzani in Qarabadeene Qadri, etc., during the compounding of aphrodisiac formulations. Mudabbar Kharekhasak (MKK) used in mentioned in Al-Qarabadeene was evaluated in this work.

Methods: Mudabbar/Tadbeer process was carried out by blending fresh KK. Juice with powdered dry KK and drying it under the sun. Juice used for process is thrice the weight of dry KK powder. The KK before and after the process was evaluated using physicochemical tests: powder characterization, extractive value, alcohol and water soluble matter, ash value, loss on drying (LOD) at 105°C, pH, high-performance thin layer chromatography (HPTLC) fingerprinting, and diosgenin content.

Results: Powder characterizations were set in. Increase in successive and nonsuccessive extractive values in various solvents, water/alcohol-soluble content, total ash, acid-insoluble ash, water-soluble ash, and sulfated ash of MKK was noted in comparison with KK. Decrease in LOD at 105°C and pH of MKK powder was observed. HPTLC fingerprinting data were developed for the identification and evaluation. Quantification of diosgenin content increased to 432.1 g/g in MKK as compared to 144.5 g/g in KK, suggesting significant increase in saponin content.

Conclusion: Data obtained clearly indicated changes in MKK validating the classical Mudabbar process, probably to enhance/modify the action of drug. Standards for crude and MKK were established for future reference.

Summary: Mudabbar process on Linn (KK) havebeen validated.Physicochemical data for Mudabbar and non mudabbar Kharekhasak (KK) powder have been set in.Diosgenin content was increased significantly in mudabbar KK. KK: Kharekhasak, TT: , MKK: mudabbar Kharekhasak, SK: , LOD: loss of weight on drying, HPTLC: High performance thin layer chromatography, BSS: British standard sieve, μl: microliter, SEM: Standard error of mean, nm: nanometer, g: gram.