[Effects of LncRNA on Proliferation of Human Colorectal Cancer SW620 Cells].

Objective: To determine the expression level of long non-coding RNA (lncRNA) imprinted maternallyexpressed transcript () in colorectal cancer tissues and its effect on proliferation of colorectal cancer SW620 cells.

Methods: Real-time quantitative PCR (qRT-PCR) was applied to detect the expression of in 20 paired tumor tissues and adjacent normal tissues,and in normal NCM460 cells and colorectal cancer SW480,HCT116 and SW620 cells. The specific small interfering RNA for (si- group) or negative control sequence (si-NC group) were transfected into SW620 cells. Proliferation of the transfected cells was detected using flow cytometry,CCK8 assay and clone formation experiment. The expressions of CyclinD1 and cyclin dependent kinase 4 (CDK4) were detected by qRT-PCR and Western blot.

Results: The expression levels of in colorectal cancer tissues and cells were higher compared with those in adjacent normal tissues and normal NCM460 cells. Lower level,cell activities and cell clone numbers were found in si- transfected cells compared with those in si-NC transfected cells ( <0.05). si-transfected cells had decreased expression of CyclinD1 and CDK4 ( <0.05).

Conclusion: expression in colorectal cancer is high. Knock-down expression can inhibit proliferation of colorectal cancer cells,which provides a potential strategy for targeted therapy of colorectal cancer.