AI Article Synopsis
- Desmosomes are important structures that help cells stick together and support their shape by anchoring intermediate filaments.
- Loss of the protein 14-3-3γ in HCT116 cells causes problems in forming desmosomes because it reduces the transport of another protein, Plakoglobin (PG), to where it's needed at the cell edge.
- Researchers found that they could fix this issue by attaching PG to another protein (EGFP-f), showing that 14-3-3γ plays a key role in moving PG to the cell border to help start desmosome formation.
Article Abstract
Desmosomes are cell-cell adhesion junctions that anchor intermediate filaments. Loss of 14-3-3γ in HCT116 cells led to defects in desmosome assembly due to a decrease in the transport of Plakoglobin (PG) to the cell border thus disrupting desmosome formation. Desmosome formation in cells lacking 14-3-3γ was restored by artificially localizing PG to the cell border by fusing it to EGFP-f (PG-EGFP-f). These results suggest that a major role of 14-3-3γ in desmosome assembly is to transport PG to the cell border leading to the initiation of desmosome formation.
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| http://dx.doi.org/10.1016/j.bbrc.2017.12.080 | DOI Listing |
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