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The influence of xeno-free culture conditions on the angiogenic and adipogenic differentiation properties of adipose tissue-derived stem cells.
Regen Ther
June 2024
Department of Medical and Translational Biology, Umeå University, SE-901 87 Umeå, Sweden.
Introduction: Before performing cell therapy clinical trials, it is important to understand how cells are influenced by different growth conditions and to find optimal xeno-free medium formulations. In this study we have investigated the properties of adipose tissue-derived stem cells (ASCs) cultured under xeno-free conditions.
Methods: Human lipoaspirate samples were digested to yield the stromal vascular fraction cells which were then seeded in i) Minimum Essential Medium-α (MEM-α) supplemented with 10 % (v/v) fetal bovine serum (FBS), ii) MEM-α supplemented with 2 % (v/v) human platelet lysate (PLT) or iii) PRIME-XV MSC expansion XSFM xeno-free, serum free medium (XV).
Utility of 3D facial reconstruction for forensic identification: a focus on facial soft tissue thickness and customized techniques.
Forensic Sci Med Pathol
January 2025
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Facial reconstruction, a crucial method in forensic identification, finds particular significance in cases where conventional means of identification are unavailable. This study addresses a significant gap in the field of forensic facial reconstruction focusing on facial soft tissue thickness (FSTT) and facial reconstruction techniques specifically tailored to the Thai population. By developing and implementing the 3D (three-dimensional) facial reconstruction program and compiling an extensive dataset of FSTT, this research makes substantial progress in advancing forensic facial reconstruction methodologies employing the combination Manchester Method, 3D skull images obtained through cone beam computed tomography (CBCT) scans were reconstructed using Autodesk Maya software.
View Article and Find Full Text PDFReconstruction of a rostral maxillary defect by two custom-made titanium implants following a partial maxillectomy for treatment of squamous cell carcinoma in a dog.
Vet Res Commun
January 2025
Veterinary Teaching Hospital, Complutense University of Madrid, Avda. Puerta de Hierro s/n, Madrid, 28040, Spain.
This case report describes the reconstruction of a rostral maxillary defect by two custom-made titanium implants following a rostral partial maxillectomy for treatment of squamous cell carcinoma (SCC) in a seven-year-old dog. An incisional biopsy and CT scan were performed to establish the diagnosis, to plan possible surgery, and to assess the margins of the tumour. The patient had no radiographic signs of metastasis at the time of diagnosis.
View Article and Find Full Text PDFIFNγ regulates ferroptosis in KFs by inhibiting the expression of SPOCD1 through DNMT3A.
Cell Death Discov
January 2025
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200011, China.
Keloid is benign skin tumor, and their curing is relatively difficult due to the unclear mechanism of formation. Inducing ferroptosis of keloid fibroblasts (KFs) may become a new method for treating keloid. Here, we discover interferon (IFN)γ could induce KFs ferroptosis through inhibiting SPOC domain-containing protein 1 (SPOCD1), serving as a mode of action for CD8T cell (CTL)-mediated keloid killing.
View Article and Find Full Text PDFMen and women's waking patterns to infant crying: Preparenthood differences are insufficient to explain uneven sharing of nighttime care.
Emotion
January 2025
Department of Culture, Cognition and Computation, Interacting Minds Center, Aarhus University.
Crying in infancy is an important emotional signal that elicits care from adults, and women are often assumed to be more sensitive and reactive to infant crying than men. In a series of studies, we tested whether preparenthood gender differences in sensitivity to infant cries are a potential driver of the unequal share of early parenting. In Study 1, we tested for differences in men and women's awakening to infant crying and alarms among nonparents in an overnight experiment ( = 142).
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