Proper recognition and tracking of microscopic sperm cells in video images are vital steps of male infertility diagnosis and treatment. The segmentation and detection of sperms in microscopic image analysis is a complicate process as a result of their small sizes, fast movements, and considerable collisions. Histogram-based thresholding schemes are very popular for this purpose, since they are quite fast and provide almost acceptable results. This paper proposes a combined method for sperm cells detection, which consists of a non-linear pre-processing stage, a histogram-based thresholding algorithm, and a tracking method based on an adaptive distance scheme. The results of conducted experiments verify the superiority of the proposed scheme with incorporated Kittler algorithm compared to the other competitive methods in the majority of cases.
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http://dx.doi.org/10.1016/j.cmpb.2017.11.005 | DOI Listing |
Life Metab
August 2022
Guangdong Laboratory for Lingnan Modern Agriculture, National Engineering Research Center for Breeding Swine Industry and Guangdong Province Key Laboratory of Animal Nutritional Regulation, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
Infertility is a global concern attributed to genetic defects, lifestyle, nutrition, and any other factors that affect the local metabolism and niche microenvironment of the reproductive system. 2-Oxoglutarate receptor 1 (OXGR1) is abundantly expressed in the testis; however, its cellular distribution and biological function of OXGR1 in the male reproductive system remain unclear. In the current study, we demonstrated that OXGR1 is primarily expressed in epididymal smooth muscle cells (SMCs).
View Article and Find Full Text PDFMol Reprod Dev
January 2025
Department of Anatomy, Histology, Forensic Medicine and Orthopedic, Section of Histology, Sapienza University of Rome, Rome, Italy.
A role for the plasminogen activator (PA) system has been postulated in mammalian gonads, considering the complex process of morphogenesis these organs undergo during their development. Our results show that mouse Sertoli cells under basal conditions produce both types of PA, tissue-type PA (tPA) and urokinase-type PA (uPA), and hormonal treatments increase the production of both enzymes. The increased enzyme secretion does not correlate with a parallel increase in their mRNAs.
View Article and Find Full Text PDFCurr Top Dev Biol
January 2025
School of Molecular Biosciences, Washington State University, Pullman, Washington, United States. Electronic address:
For mammalian spermatogenesis to proceed normally, it is essential that the population of testicular progenitor cells, A undifferentiated spermatogonia (A), undergoes differentiation during the A to A1 transition that occurs at the onset of spermatogenesis. The commitment of the A population to differentiation and leaving a quiescent, stem-like state gives rise to all the spermatozoa produced across the lifespan of an individual, and ultimately determines male fertility. The action of all-trans retinoic acid (atRA) on the A population is the determining factor that induces this change.
View Article and Find Full Text PDFPLoS One
January 2025
Laboratory of Developmental Biology, Department of Morphology and Genetics-Paulista Medicine School, Federal University of Sao Paulo (UNIFESP), Sao Paulo, SP, Brazil.
Melatonin is a pineal hormone synthesized exclusively at night, in several organisms. Its action on sperm is of particular interest, since they transfer genetic and epigenetic information to the offspring, including microRNAs, configuring a mechanism of paternal epigenetic inheritance. MicroRNAs are known to participate in a wide variety of mechanisms in basically all cells and tissues, including the brain and the sperm cells, which are known, respectively, to present 70% of all identified microRNAs and to transfer these molecules to the embryo.
View Article and Find Full Text PDFNucleic Acids Res
January 2025
Institute of Reproductive Medicine, School of Medicine, Nantong University, Nantong 226001, China.
Chromatin remodeling, which involves the histone-to-protamine exchange process during spermiogenesis, is crucial for sperm nuclear condensation and male fertility. However, the key regulators and underlying molecular mechanisms involved in this process remain largely unexplored. In this study, we discovered that deficiency in the family with sequence similarity 170 member A (Fam170a) led to abnormal sperm nuclear morphology and male infertility in mice, mirroring the observation of very low Fam170a transcription levels in sperm of infertile men with teratozoospermia.
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