AI Article Synopsis
- The article discusses the significance of 1,3-dipolar cycloadditions of azides and alkynes in both biology and chemistry, emphasizing their widespread use.
- It addresses the challenges posed by the need for a copper catalyst in these reactions and presents a solution through the development of a copper-free microwave-assisted process.
- The researchers successfully synthesized various triazoles and applied this new method to bioconjugate a full-length protein using unnatural amino acids, marking a pioneering achievement in microwave-mediated protein chemistry.
Article Abstract
The prevalence of 1,3-dipolar cycloadditions of azides and alkynes within both biology and chemistry highlights the utility of these reactions. However, the use of a copper catalyst can be prohibitive to some applications. Consequently, we have optimized a copper-free microwave-assisted reaction to alleviate the necessity for the copper catalyst. A small array of triazoles was prepared to examine the scope of this approach, and the methodology was translated to a protein context through the use of unnatural amino acids to demonstrate one of the first microwave-mediated bioconjugations involving a full length protein.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761740 | PMC |
| http://dx.doi.org/10.1016/j.bmcl.2017.12.007 | DOI Listing |
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