Autophagy is essential in lens organelle degradation. This study aimed to seek potential autophagy-associated long noncoding RNAs (lncRNAs) and their relative mechanisms in human lens development using the "fried egg" lentoid body (LB) generation system. The expression pattern of LC3B in differentiating LBs was similar to that in developing a mouse lens in vivo. Among the massive lncRNAs expressed with a significant difference between induced pluripotent stem cells (iPSCs) and LBs, lncRNA affecting LC3B (ALB), which was predicted to have a co-relationship with the autophagy marker LC3B, was highly expressed in differentiating lens fibers in LBs. This result was consistent with its high expression in human embryonic lenses compared to those in embryonic stem cells (ESCs). Furthermore, lncRNA ALB knockdown resulted in the downregulation of LC3BII at the protein level, therefore inhibiting the autophagy process in human lens epithelial cells (HLECs). Our results identify lncRNA ALB, a potential autophagy regulator in organelle degradation during human lens development, highlighting the importance of lncRNAs in lens development.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650653 | PMC |
| http://dx.doi.org/10.1016/j.omtn.2017.09.011 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring the Role of Immersive Virtual Reality Simulation in Health Professions Education: Thematic Analysis.
JMIR Med Educ
March 2025
Division of Pulmonary, Critical Care, & Sleep Medicine, Department of Medicine, NYU Grossman School of Medicine, 550 First Avenue, 15th Floor, Medical ICU, New York, NY, 10016, United States, 1 2122635800.
Background: Although technology is rapidly advancing in immersive virtual reality (VR) simulation, there is a paucity of literature to guide its implementation into health professions education, and there are no described best practices for the development of this evolving technology.
Objective: We conducted a qualitative study using semistructured interviews with early adopters of immersive VR simulation technology to investigate use and motivations behind using this technology in educational practice, and to identify the educational needs that this technology can address.
Methods: We conducted 16 interviews with VR early adopters.
Differences between children and young adults in the effects of difficulty and value of learning items on cognitive offloading strategies.
Psychol Res
March 2025
School of Education, Guangzhou University, Guangzhou, 510006, People's Republic of China.
Cognitive offloading refers to the use of external tools to assist in memory processes.This study investigates the effects of item difficulty and value on cognitive offloading during a word-pair learning task, comparing children and young adults in a context where both cues coexist. In Experiment 1, we examined the impact of difficulty and value cues on cognitive offloading using a 2 (difficulty: easy vs.
View Article and Find Full Text PDFA Novel Model for Cornea Power Shift in Eyes Wearing Orthokeratology Lenses With Different Back Optic Zone Diameters for Myopia Control.
Invest Ophthalmol Vis Sci
March 2025
College of Optometry, Nova Southeastern University, Davie, Florida, United States.
Purpose: The purpose of this study was to quantify the corneal power changes after wearing orthokeratology lenses of different back optic zone diameters (BOZDs) and to propose a novel 4-parameter model capable of revealing the associations between each parameter and axial length growth (ALG).
Methods: A prospective self-controlled study was conducted between June 2022 and December 2023. One eye in each subject (N = 33) was randomly assigned to wear a lens with a BOZD of either 5 mm (5 oz) or 6 mm (6 oz).
The Microbiome in Asthma Heterogeneity: The Role of Multi-Omic Investigations.
Immunol Rev
March 2025
Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Asthma is one of the most prevalent and extensively studied chronic respiratory conditions, yet the heterogeneity of asthma remains biologically puzzling. Established factors like exogenous exposures and treatment adherence contribute to variability in asthma risk and clinical outcomes. It is also clear that the endogenous factors of genetics and immune system response patterns play key roles in asthma.
View Article and Find Full Text PDFTreating Situational Couple Aggression: An Integrative Behavioral Couple Therapy Approach.
Fam Process
March 2025
Department of Psychology, University of Oregon, Eugene, USA.
Situational couple aggression is common; most couples who seek conjoint treatment report at least some instances of aggression. Modern couple therapy research provides support for the safe and effective use of conjoint treatment with situationally aggressive couples; however, many clinicians feel ill equipped to assess and treat aggression via relational therapy. We propose that integrative behavioral couple therapy (IBCT) offers a uniquely suited lens for working with couples who participate in situational aggression.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!