A novel fluorescent receptor L was synthesized by Schiff base condensation of 1-pyrenemethylamine with the vitamin B cofactor pyridoxal. The receptor L is highly selective and sensitive towards Zn ions among other tested metal ions. Upon interaction with Zn, the receptor L showed a distinct fluorescence enhancement at 485 nm due to the excimer formation leading to the fluorescent colour change from blue to bluish-green. Subsequently, when the in situ generated ZnL complex interacted with various anions and amino acids, the addition of HPO and cysteine reinstated the fluorescence of the receptor L due to the demetalation of Zn from the ZnL complex. Accordingly, the receptor L was developed for the highly selective, specific and sensitive detection of three important bioactive analytes, i.e., Zn, HPO and cysteine with a detection limit down to 2.3 × 10 M, 2.18 × 10 M and 1.59 × 10 M, respectively. Additionally, the receptor L was applied to the detection of intracellular Zn ions in live HeLa cells.
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