Evaluation of colorimetric assays for determination of HOin planta during fungal wood decomposition.

J Microbiol Methods

Department of Plant & Microbial Biology, University of Minnesota, Saint Paul, MN 55108, USA. Electronic address:

Published: February 2018

AI Article Synopsis

  • Hydrogen peroxide (HO) is important for fungi to break down plant cell walls by generating oxygen radicals.
  • * The levels of hydrogen peroxide in plants are usually low during decomposition, making them hard to detect with standard methods.
  • * The study evaluated various detection methods to find the most effective one based on sensitivity and selectivity.

Article Abstract

Hydrogen peroxide (HO) plays a critical role in generating oxygen radicals as fungi attack and deconstruct plant cell walls. Its concentrations in planta, however, are often low during decomposition and evade detection by traditional methods. Here, we compared relevant methods and selected the best based on detection limits and selectivity.

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http://dx.doi.org/10.1016/j.mimet.2017.12.004DOI Listing

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