AI Article Synopsis
- The study investigates how genistein, an isoflavone, may counteract lung issues caused by estrogen deficiency in ovariectomized diabetic rats, hypothesizing that it could reverse harmful protein expression changes.
- Wistar female rats were divided into four groups to assess the effects of ovariectomy, high-fat diets, and genistein treatment on lung health, using various evaluation techniques including Western blotting and histological staining.
- Results showed that genistein reduced inflammatory and apoptotic markers and helped maintain healthy lung structure, suggesting its potential as a natural alternative therapy for addressing estrogen deficiency effects in diabetic menopausal women.
Article Abstract
Objectives: The role of isoflavones in pulmonary structure and function during menopause is not well studied. Moreover, the important role of estrogen in the physiological function of respiratory system has been revealed. Genistein, as an isoflavone, mimics estrogenic in diabetic and ovariectomized rats. Here, we hypothesized that genistein would reverse changes in the protein expression levels related to estrogen deficiency in the lung of ovariectomized diabetic rats.
Materials And Methods: Wistar female rats were assigned to four experimental groups (n=10 in each group): sham, rats underwent laparotomy without removing the ovaries; OVX, rats that underwent ovariectomy; OVX.D, rats underwent bilateral ovariectomy and were fed a high-fat diet (HFD); OVX.D.G, ovariectomized diabetic rats with genistein administration (1 mg/kg /day). After ovariectomy, rats continued to feed HFD for a 4-week period. After 4 weeks of HFD feeding, a single dose of 30 mg/kg of streptozotocin was administered in the diabetic group. Genistein was administered for eight weeks. At the end of the experiment, lung tissue was removed and Western blotting technique and hematoxylin-eosin staining were used for evaluation of the lung.
Results: Treatment with genistein significantly decreased inflammatory and apoptotic biomarkers in the ovariectomized diabetic rats compared to non-treated animals (<0.05). Also, genistein exerted a protective effect in the lung architecture.
Conclusion: Genistein partly reversed ovariectomy-induced changes in apoptotic and inflammatory biomarkers in the lung. Our data suggest that genistein treatment as a natural replacement therapy may prevent the estrogen deficiency effects in the lung of diabetic menopausal women.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5722990 | PMC |
| http://dx.doi.org/10.22038/IJBMS.2017.9599 | DOI Listing |
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