Calcineurin modulates environmental stress survival and virulence of the human fungal pathogen Previously, we identified 44 putative calcineurin substrates, and proposed that the calcineurin pathway is branched to regulate targets including Crz1, Pbp1, and Puf4 in In this study, we characterized Had1, which is one of the putative calcineurin substrates belonging to the ubiquitously conserved haloacid dehalogenase β-phosphoglucomutase protein superfamily. Growth of the ∆ mutant was found to be compromised at 38° or higher. In addition, the ∆ mutant exhibited increased sensitivity to cell wall perturbing agents, including Congo Red and Calcofluor White, and to an endoplasmic reticulum stress inducer dithiothreitol. Virulence studies revealed that the mutation results in attenuated virulence compared to the wild-type strain in a murine inhalation infection model. Genetic epistasis analysis revealed that Had1 and the zinc finger transcription factor Crz1 play roles in parallel pathways that orchestrate stress survival and fungal virulence. Overall, our results demonstrate that Had1 is a key regulator of thermotolerance, cell wall integrity, and virulence of .
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http://dx.doi.org/10.1534/g3.117.300444 | DOI Listing |
Naunyn Schmiedebergs Arch Pharmacol
December 2024
Department of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Researchers have repurposed several existing anti-inflammatory drugs as potential antifungal agents in recent years. So, this study aimed to investigate the effects of anti-inflammatory drugs on the growth, biofilm formation, and expression of genes related to morphogenesis and pathogenesis in Candida albicans. The minimum inhibitory concentration (MIC) of anti-inflammatory drugs was assessed using the broth microdilution method.
View Article and Find Full Text PDFJ Morphol
January 2025
Department of Invertebrate Zoology, Saint Petersburg State University, Saint Petersburg, Russian Federation.
The colonial system of integration (CSI) provides intracolonial nutrient supply in many gymnolaemate bryozoans. In Ctenostomata, its presence is known for species with stolonal colonies, for example, vesicularioideans, but its structure is almost unexplored. The CSI is thought to be absent in alcyonidioideans and other ctenostomes.
View Article and Find Full Text PDFBackground And Aims: Since salinity stress may occur across stages of rice (Oryza sativa L.) crop growth, understanding the effects of salinity at reproductive stage is important although it has been much less studied than at seedling stage.
Methods: In this study, lines from the Rice Diversity Panel 1 (RDP1) and the 3000 Rice Genomes (3KRG) were used to screen morphological and physiological traits, map loci controlling salinity tolerance through genome-wide association studies (GWAS), and identify favorable haplotypes associated with reproductive stage salinity tolerance.
Evodevo
December 2024
Institute of Botany, Justus-Liebig-University, Heinrich-Buff-Ring 38, 35392, Giessen, Germany.
Background: Fruits, with their diverse shapes, colors, and flavors, represent a fascinating aspect of plant evolution and have played a significant role in human history and nutrition. Understanding the origins and evolutionary pathways of fruits offers valuable insights into plant diversity, ecological relationships, and the development of agricultural systems. Arabidopsis thaliana (Brassicaceae, core eudicot) and Eschscholzia californica (California poppy, Papaveraceae, sister group to core eudicots) both develop dry dehiscent fruits, with two valves separating explosively from the replum-like region upon maturation.
View Article and Find Full Text PDFBMC Microbiol
December 2024
Department of Clinical Laboratory, Shanghai Eighth People's Hospital, No.8 Caobao Road, Xuhui District, Shanghai, 200235, P.R. China.
Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant public health problem. This study investigated the antimicrobial properties and mechanisms of berberine (BBR), a plant alkaloid, against MRSA, evaluating its potential to enhance antibiotic therapy.
Results: Berberine only demonstrated variable but significant inhibitory effects on 50 clinical MRSA strains.
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