AI Article Synopsis
- The maturation of porcine oocytes is critical for successful fertilization, but the number of fully mature oocytes remains low.
- Researchers studied cell adhesion in porcine oocytes collected from 45 gilts and performed tests to analyze changes before and after in vitro maturation (IVM).
- The study found significant down-regulation of genes related to adhesion processes after IVM, suggesting that biological adhesion is important for oocyte maturation and identifying potential new molecular markers for this process.
Article Abstract
Proper maturation of the mammalian oocyte is a compound processes determining successful monospermic fertilization, however the number of fully mature porcine oocytes is still unsatisfactory. Since oocytes' maturation and fertilization involve cellular adhesion and membranous contact, the aim was to investigate cell adhesion ontology group in porcine oocytes. The oocytes were collected from ovaries of 45 pubertal crossbred Landrace gilts and subjected to two BCB tests. After the first test, only granulosa cell-free BCB⁺ oocytes were directly exposed to microarray assays and RT-qPCR ("before IVM" group), or first in vitro matured and then if classified as BCB⁺ passed to molecular analyses ("after IVM" group). As a result, we have discovered substantial down-regulation of genes involved in adhesion processes, such as: organization of actin cytoskeleton, migration, proliferation, differentiation, apoptosis, survival or angiogenesis in porcine oocytes after IVM, compared to oocytes analyzed before IVM. In conclusion, we found that biological adhesion may be recognized as the process involved in porcine oocytes' successful IVM. Down-regulation of genes included in this ontology group in immature oocytes after IVM points to their unique function in oocyte's achievement of fully mature stages. Thus, results indicated new molecular markers involved in porcine oocyte IVM, displaying essential roles in biological adhesion processes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751287 | PMC |
| http://dx.doi.org/10.3390/ijms18122685 | DOI Listing |
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