AI Article Synopsis
- The text describes a dataset created using gadoteridol-derivatized lysozyme (gadolinium lysozyme) for a specific technique called two-colour serial femtosecond crystallography (SFX).
- This dataset was collected at the SACLA facility, utilizing a double-pulse beam to capture two diffraction patterns at once, achieving a resolution of 1.9 Å.
- The data is publicly accessible via the Coherent X-ray Imaging Data Bank to assist in developing algorithms for further research.
Article Abstract
We provide a detailed description of a gadoteridol-derivatized lysozyme (gadolinium lysozyme) two-colour serial femtosecond crystallography (SFX) dataset for multiple wavelength anomalous dispersion (MAD) structure determination. The data was collected at the Spring-8 Angstrom Compact free-electron LAser (SACLA) facility using a two-colour double-pulse beam to record two diffraction patterns simultaneously in one diffraction image. Gadolinium lysozyme was chosen as a well-established model system that has a very strong anomalous signal. Diffraction patterns from gadolinium lysozyme microcrystals were recorded to a resolution of 1.9 Å in both colours. This dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5726314 | PMC |
| http://dx.doi.org/10.1038/sdata.2017.188 | DOI Listing |
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