AI Article Synopsis

  • Oestrogen (E2) increases FOXP3 expression in regulatory T cells through its receptor ERα, crucial for T cell function in cervical cancer.
  • Blocking ERα in T cells decreases FOXP3 levels and impairs their function in tumor environments.
  • The study reveals that E2:ERα complexes directly interact with the FOXP3 promoter, demonstrating the importance of E2 in regulating T cell physiology in cervical cancer.

Article Abstract

Oestrogen controls Foxp3 expression in regulatory T cells (T cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T cells (CD4CD25CD127), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T cells. Chromatin immunoprecipitation analyses of male blood T cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T cell physiology.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725534PMC
http://dx.doi.org/10.1038/s41598-017-17102-wDOI Listing

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