Sequencing of Hot Spot 1 from Saprochaete capitata To Search for a Relationship to Reduced Echinocandin Susceptibility.

, formerly known as , is an emerging fungal pathogen with low susceptibility to echinocandins. Here, we report the nucleotide sequence of the hot spot 1 region of the gene ( HS1), which codifies for the catalytic subunit of β-1,3-d-glucan synthase, the target of echinocandins. For that purpose, we first designed degenerated oligonucleotide primers derived from conserved flanking regions of the HS1 segment of 12 different fungal species. Interestingly, analysis of the translated HS1 sequences of 12 isolates of revealed that all of them exhibited the same F-to-L substitution in a position that is highly related to reduced echinocandin susceptibility.