We are witnessing a breathtaking development in light (fluorescence) microscopy, where structures can be resolved down to the size of a ribosome within cells. This has already yielded surprising insight into the subcellular structure of cells, including the smallest cells, bacteria. Moreover, it has become possible to visualize and track single fluorescent protein fusions in real time, and quantify molecule numbers within individual cells. Combined, super resolution and single molecule tracking are pushing the limits of our understanding of the spatio-temporal organization even of the smallest cells to an unprecedented depth.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mib.2017.11.027 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development and experimental validation of a machine learning model for the prediction of new antimalarials.
BMC Chem
January 2025
Department of Pharmacy, Birla Institute of Technology and Science Pilani, Pilani Campus, Vidya Vihar, Pilani, Rajasthan, 333 031, India.
A large set of antimalarial molecules (N ~ 15k) was employed from ChEMBL to build a robust random forest (RF) model for the prediction of antiplasmodial activity. Rather than depending on high throughput screening (HTS) data, molecules tested at multiple doses against blood stages of Plasmodium falciparum were used for model development. The open-access and code-free KNIME platform was used to develop a workflow to train the model on 80% of data (N ~ 12k).
View Article and Find Full Text PDFBiomarker analysis from complex biofluids by an on-chip chemically modified light-controlled vertical nanopillar array device.
Nat Protoc
January 2025
Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv-Yafo, Israel.
Nanostructured devices have proven useful in a broad range of applications, from diagnosing diseases to discovering and screening new drug molecules. We developed vertical silicon nanopillar (SiNP) arrays for on-chip multiplex capture of selected biomolecules using a light-induced release of the array's selectively captured biomarkers. This platform allows the rapid, reusable and quantitative capture and release of a selection of biomarkers, followed by their downstream analysis.
View Article and Find Full Text PDFRetinal Phenotypes and Single-cell Sequencing Analysis of Ush2a Knockout Mice.
Exp Eye Res
January 2025
Shenzhen Eye Hospital, Jinan University, Shenzhen Eye Institute, Shenzhen, 518040 Guangdong, China. Electronic address:
Usher syndrome is a rare autosomal recessive genetic disorder that primarily affects both vision and hearing, manifesting as sensorineural hearing loss and progressive vision loss caused by retinitis pigmentosa. The pathogenesis of retinal degeneration in Usher syndrome is still largely unknown. In this study, a novel Ush2a knockout mouse model was successfully constructed using CRISPR/Cas9 technology.
View Article and Find Full Text PDFReliable and specific biosensing on single- and double-stranded aptamer functionalized remote dual-gate organic field-effect transistors: A comparison.
Talanta
January 2025
College of Science, Nanjing Forestry University, Nanjing, 210037, China. Electronic address:
Organic field-effect transistors (OFETs) integrated with commercial transistors are promising sensing platforms characterized by enhanced device uniformity, functional diversity, and electrical output stability. Aptamers with charged backbones and a high affinity for target molecules are anticipated to mitigate the limitations imposed by Debye screening in physiological environments with high ionic strength, thereby facilitating specific biological recognition in complex surroundings. This study presents two reliable OFET aptasensors for vascular endothelial growth factor (VEGF) and offers a systematic comparison of their performance.
View Article and Find Full Text PDFEpithelial cell diversity and immune remodeling in bladder cancer progression: insights from single-cell transcriptomics.
J Transl Med
January 2025
Department of Urology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
Background: The progression of bladder cancer (BC) from non-muscle-invasive bladder cancer (NMIBC) to muscle-invasive bladder cancer (MIBC) significantly increases disease severity. Although the tumor microenvironment (TME) plays a pivotal role in this process, the heterogeneity of tumor cells and TME components remains underexplored.
Methods: We characterized the transcriptomes of single cells from 11 BC samples, including 4 NMIBC, 4 MIBC, and 3 adjacent normal tissues.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!