AI Article Synopsis
- Breast cancer is made up of various subtypes with different genetic and clinical characteristics, which this study highlights by detailing a method for long-term culturing of human mammary epithelial organoids.
- Over 100 organoid lines were created from primary and metastatic breast cancer, effectively mirroring the original tumors' features like histopathology and hormone receptor status.
- The organoids were also shown to maintain genetic consistency and allow for drug testing that corresponds well with actual patient outcomes, making them valuable for cancer research and personalized treatment strategies.
Article Abstract
Breast cancer (BC) comprises multiple distinct subtypes that differ genetically, pathologically, and clinically. Here, we describe a robust protocol for long-term culturing of human mammary epithelial organoids. Using this protocol, >100 primary and metastatic BC organoid lines were generated, broadly recapitulating the diversity of the disease. BC organoid morphologies typically matched the histopathology, hormone receptor status, and HER2 status of the original tumor. DNA copy number variations as well as sequence changes were consistent within tumor-organoid pairs and largely retained even after extended passaging. BC organoids furthermore populated all major gene-expression-based classification groups and allowed in vitro drug screens that were consistent with in vivo xeno-transplantations and patient response. This study describes a representative collection of well-characterized BC organoids available for cancer research and drug development, as well as a strategy to assess in vitro drug response in a personalized fashion.
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| http://dx.doi.org/10.1016/j.cell.2017.11.010 | DOI Listing |
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