AI Article Synopsis
- The study focused on synthesizing and characterizing small gold nanoparticles (<5 nm) coated with Bovine Serum Albumin (BSA) using various techniques, including UV-vis spectrophotometry and electron microscopy.
- It tested the effectiveness of these nanoparticles on three cancer cell lines (Rhabdomyosarcoma, Murine fibroblast, and macrophages) using a photothermal therapy approach with different concentrations and laser irradiation times.
- Results showed significant cytotoxicity, especially in the Rhabdomyosarcoma cell line, where over 74% cell death was observed at a concentration of 1 mg/ml with 3 minutes of laser exposure, highlighting the potential of BSA-AuNPs for
Article Abstract
In the present study, small gold nanoparticles <5 nm coated with natural protein Bovine Serum Albumin (BSA) was synthesized and characterized using UV-vis spectrophotometer, Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), zeta potential and scanning electron microscopy (SEM). Three types of cancer cell lines; Rhabdomyosarcoma (RD), Murine fibroblast (L20B) and RAW 264.7 monocyte-macrophage (MQ) were tested and treated by photothermal strategy, in vitro, by conjugating BSA-AuNPs complex of (0.125, 0.25, 0.5 and 1 mg/ml) concentrations with continuous low power laser irradiation, green (532 nm) and near-infrared (NIR) (800 nm) at 0.5, 1, 2 and 3 min, separately. Cytotoxicity effect was determined by MTT assay. The vital impact of photothermal technique has investigated at 1 mg/ml and 3 min irradiation period as identified in RD cell line in comparison with other types; where cytotoxicity more than 74% was reached. Prominent results were demonstrated in the green and NIR region by pH -induced aggregation effect of small nanoparticles inside the cancer cells, which make the small-sized BSA-AuNPs are promising agents for cancer photothermal therapy.
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| http://dx.doi.org/10.1016/j.pdpdt.2017.12.004 | DOI Listing |
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