Experimental and field studies of different turtle species suggest that moisture influences embryonic development and sex ratios, wetter substrates tend to produce more males, and drier substrates produce more females. In this study, we used Trachemys scripta elegans to test the effect of moisture on embryonic development and sex ratios. T. s. elegans eggs were incubated under different temperature and moisture regimes. We monitored embryonic development until stage 22 (after sex determination) and, for the first time, we estimated sex ratios using a male-specific transcriptional molecular marker, Sox9. Among treatments, we found differences in developmental rates, egg mass, and sex ratio. Embryos developed slowly in cooler and wetter sand substrate while water uptake by the eggs was significantly greater on wetter substrates. Developmental differences were due to moisture interacting with temperature where increased water content of the sand resulted in temperatures that were 2-3°C lower than air temperatures. The coolest and the wettest substrates produced 100% males compared to 42% males from the warmest and driest treatment. Further, we found that embryonic growth appears to be more sensitive to temperature at earlier stages of development and to moisture at later stages. This study shows how moisture may change the incubation conditions inside nests by changing the temperature experienced by eggs, which affects development, growth and sex ratios. The results of this study highlight the importance of including moisture conditions when predicting embryo growth and sex ratios and in developing proxies of embryonic development.
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http://dx.doi.org/10.1016/j.zool.2017.11.009 | DOI Listing |
Purpose: The purpose of this study is to describe the inter- and intra-individual differences in the platelet concentration between blood and platelet-rich plasma (PRP) preparation, assess intersubject differences considering demographic and anthropometric variables, describe PRP code distribution and analyse intrasubject variability.
Methods: A retrospective analysis was conducted using a single-centre patient database from November 2021 to November 2023. It included patients with musculoskeletal pathologies treated with PRP injections.
J Migr Health
December 2024
Institute of Public Health of Chile (ISP), Santiago, Chile.
Background: International migrants are central to HIV research, but comparative data on their infection rates versus resident populations, including in Chile, are scarce. This study compares HIV incidence rates between international migrants and Chileans.
Methods: A longitudinal study was conducted.
Background: Lipid Accumulation Product (LAP), which is derived from measurements of waist circumference and triglyceride (TG) levels, serves as a comprehensive indicator of lipid accumulation. Emerging research indicates that lipid accumulation dysfunction might significantly contribute to the pathogenesis of Chronic Obstructive Pulmonary Disease (COPD). Nevertheless, the investigation into the association between LAP and COPD risk is still insufficient, particularly in population-based research.
View Article and Find Full Text PDFFront Nutr
January 2025
Shenzhen Bao'an Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, China.
Objectives: Despite substantial evidence that visceral obesity is an epidemiological risk factor for hyperuricemia (HUA), studies on the connection between the Metabolic Score for Visceral Fat (METS-VF) and HUA remain insufficient. This research focused on METS-VF's potential role as a risk factor for HUA.
Methods: Notably, 8,659 participants from the National Health and Nutrition Examination Survey (NHANES) from 1999 to 2018 were enrolled in this study.
Drug Alcohol Depend Rep
March 2025
Department of Medicine, University of Washington, Seattle, WA, USA.
Aim: Unhealthy alcohol use is often correlated with experiences of intimate partner violence (IPV). We investigated how different types of IPV (sexual, physical, emotional, and financial) were associated with unhealthy alcohol use among women engaged in sex work in Mombasa, Kenya.
Methods: This cross-sectional study included 283 HIV-negative women who engaged in sex work recruited from an ongoing cohort study.
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