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Reproducibility and Consistency of Isolation Protocols for Fibroblasts, Smooth Muscle Cells, and Epithelial Cells from the Human Vagina.
Cells
January 2025
Reproductive Biology Laboratory, Amsterdam UMC-Location AMC, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.
(1) Background: For the reconstruction of a human vagina, various surgical procedures are available that are often associated with complications due to their failure to mimic the physiology of the human vagina. We recently developed a vascularized, organ-specific matrix from healthy human vaginal wall tissue with suitable biomechanical properties. A superior graft would require further extensive colonization with autologous vaginal cells to reduce complications upon implantation.
View Article and Find Full Text PDFBiogenic synthesized CuO nanoparticles and 5-fluorouracil loaded anticancer gel for HeLa cervical cancer cells.
Discov Nano
December 2024
Department of Pharmaceutics, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, 603203, India.
Cervical cancer remains a significant health challenge in developing countries are high due to low HPV vaccination rates, delayed diagnosis, and restricted healthcare access. Metal nanomaterials, such as copper oxide (CuO) nanoparticles (NPs), have shown significant promise in cancer therapy due to their ability to induce apoptosis. 5-Fluorouracil (5-Fu) enhances the cytotoxic effect against cervical cancer, working synergistically with CuO NPs to maximize the therapeutic impact while potentially reducing the 5-Fu's systemic side effects.
View Article and Find Full Text PDFUnveiling the neonatal gut microbiota: exploring the influence of delivery mode on early microbial colonization and intervention strategies.
Arch Gynecol Obstet
December 2024
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Article Synopsis
- Recent research highlights the vital role of neonatal gut microbiota in health and immune system development, leading to investigations into its early formation and influencing factors.
- The mode of delivery, especially the rising trend of cesarean sections, significantly impacts the establishment of gut microbiota in newborns, posing public health concerns.
- The review discusses interventions like probiotics, prebiotics, and breastfeeding to mitigate colonization issues in cesarean-born infants, aiming to improve gut microbiota development and prevent related health problems.
Evidence-based cesarean delivery: intraoperative management following placental delivery until skin closure (part 9).
Am J Obstet Gynecol MFM
November 2024
Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Thomas Jefferson University, Philadelphia, PA. Electronic address:
This expert review provides recommendations for the cesarean delivery technique after placental delivery to skin closure. After placental delivery sponge curettage may be omitted as it has not been shown to decrease the risk of retained products of conception. Uterine irrigation and mechanical cervical dilation cannot be recommended.
View Article and Find Full Text PDFImpact of vaginal seeding on the gut microbiome of infants born via cesarean section: A systematic review.
J Infect
December 2024
Research Center of China Medical University Birth Cohort, China Medical University, Shenyang 110122, China; Health Sciences Institute, China Medical University, Shenyang 110122, China; Liaoning Key Laboratory of Obesity and Glucose/Lipid Associated Metabolic Diseases, Shenyang 110122, China. Electronic address:
Objective: This systematic review summarizes eight studies involving 558 cesarean section (CS)-born infants (274 exposed to vaginal seeding (VS), 284 not exposed) and 261 infants born vaginally to investigate the effect of VS on gut microbiome colonization and development in CS-born infants.
Methods: This study adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Relevant articles published before March 6, 2024, were identified through systematic searches of PubMed, Scopus, and Web of Science.
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