Potential rat model of anxiety-like gastric hypersensitivity induced by sequential stress.

Aim: To establish a rat model of anxiety-like gastric hypersensitivity (GHS) of functional dyspepsia (FD) induced by novel sequential stress.

Methods: Animal pups were divided into two groups from postnatal day 2: controls and the sequential-stress-treated. The sequential-stress-treated group received maternal separation and acute gastric irritation early in life and restraint stress in adulthood; controls were reared undisturbed with their mothers. Rats in both groups were followed to adulthood (8 wk) at which point the anxiety-like behaviors and visceromotor responses to gastric distention (20-100 mmHg) and gastric emptying were tested. Meanwhile, alterations in several anxiety-related brain-stomach modulators including 5-hydroxytryptamine (5-HT), γ-aminobutyric acid (GABA), brain-derived neurotrophic factor (BDNF) and nesfatin-1 in the rat hippocampus, plasma and gastric fundus and the 5-HT1A receptor (5-HT1AR) in the hippocampal CA1 subfield and the mucosa of the gastric fundus were examined.

Results: Sequential-stress-treated rats simultaneously demonstrated anxiety-like behaviors and GHS in dose-dependent manner compared with the control group. Although rats in both groups consumed similar amount of solid food, the rate of gastric emptying was lower in the sequential-stress-treated rats than in the control group. Sequential stress significantly decreased the levels of 5-HT (51.91 ± 1.88 104.21 ± 2.88, < 0.01), GABA (2.38 ± 0.16 5.01 ± 0.13, < 0.01) and BDNF (304.40 ± 10.16 698.17 ± 27.91, < 0.01) in the hippocampus but increased the content of nesfatin-1 (1961.38 ± 56.89 1007.50 ± 33.05, < 0.01) in the same site; significantly decreased the levels of 5-HT (47.82 ± 2.29 89.45 ± 2.61, < 0.01) and BDNF (257.05 ± 12.89 536.71 ± 20.73, < 0.01) in the plasma but increased the content of nesfatin-1 in it (1391.75 ± 42.77 737.88 ± 33.15, < 0.01); significantly decreased the levels of 5-HT (41.15 ± 1.81 89.17 ± 2.31, < 0.01) and BDNF (226.49 ± 12.10 551.36 ± 16.47, < 0.01) in the gastric fundus but increased the content of nesfatin-1 in the same site (1534.75 ± 38.52 819.63 ± 38.04, < 0.01). The expressions of 5-HT1AR in the hippocampal CA1 subfield and the mucosa of the gastric fundus were down-regulated measured by IHC (Optical Density value: Hippocampus 15253.50 ± 760.35 21149.75 ± 834.13; gastric fundus 15865.25 ± 521.24 23865.75 ± 1868.60; < 0.05, respectively) and WB (0.38 ± 0.01 0.57 ± 0.03, < 0.01) ( = 8 in each group).

Conclusion: Sequential stress could induce a potential rat model of anxiety-like GHS of FD, which could be used to research the mechanisms of this intractable disease.