AI Article Synopsis
- * The Tubuna propolis contained rare piperidinic alkaloids and C-glycopyranoside flavonoids, while Mandaçaia's propolis was rich in terpenoids and flavonoids.
- * Both extracts showed cytotoxic effects on melanoma cells by increasing reactive oxygen species, affecting mitochondrial function, and inducing apoptosis, with Mandaçaia also causing cell cycle arrest in the G2/M phase.
Article Abstract
The present study characterized propolis extracts produced by Scaptotrigona bipunctata (Tubuna) and Melipona quadrifasciata (Mandaçaia) by LC-MS/MS; their cytotoxicity as well as the mechanism of action in a melanoma cellular model were also assessed. The chemical characterization performed by UPLC-ESI-QTOF/MS analysis revealed uncommon presence of piperidinic alkaloids in Tubuna's propolis extract together with C-glycopyranoside flavonoids. Mandaçaia's propolis collected in the same area rather presented terpenoids and flavonoids. Regarding the mechanism of cytotoxicity, propolis extracts increased the accumulation of reactive oxygen species (ROS), reduced the potential of mitochondrial membrane, induced a decrease in the proteins Bcl-2 and AKT-3 levels, and decreased melanoma cells' migration and invasion. Both propolis extracts induced apoptosis while only Mandaçaia's propolis extract induced cell cycle arrest in G2/M.
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| http://dx.doi.org/10.1016/j.jpba.2017.11.038 | DOI Listing |
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