AI Article Synopsis
- The study focuses on how Helicobacter pylori attaches to gastric cells, highlighting the potential role of sugar antigens in this process.
- The experiment used gastric cancer cells and a specific H. pylori strain to measure levels of various factors through ELISA assays.
- Results showed that while the bacteria did not significantly affect most factors, the addition of lectins led to a decrease in certain mucins and antigens, suggesting that these lectins might facilitate the binding of H. pylori to gastric tissue.
Article Abstract
Purpose: Attachment of Helicobacter pylori to the mucous epithelial cells and the mucous layer is said to be a crucial step for infection development. Sugar antigens of gastric mucins (MUC5AC, MUC1) can act as receptors for bacterial adhesins. The aim of the study was to investigate if Lotus tetragonolobus and Maackia amurensis lectins influence the level of MUC1, MUC5AC, Lewis b, H type 1, sialyl Lewis x, phospho-IκBα and interleukin 8 in Helicobacter pylori infected gastric cancer cells.
Materials And Methods: The study was performed with one clinical H. pylori strain and CRL-1739 gastric cancer cells. To assess the levels of mentioned factors immunosorbent ELISA assays were used.
Results: Coculture of cells with bacteria had no clear effect on almost all examined structures. After coculture with H. pylori and lectins, a decrease of the level of both mucins, Lewis b and H type 1 antigens was observed. Lectins addition had no effect on sialyl Lewis x. Maackia amurensis caused slight increase of phospho-IκBα while interleukin 8 level was decreased.
Conclusions: Lotus tetragonolobus and Maackia amurensis lectins can mediate in binding of Helicobacter pylori to gastric epithelium.
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| http://dx.doi.org/10.1016/j.advms.2017.06.003 | DOI Listing |
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