In vitro functional analyses of cells are widely used to investigate the molecular mechanisms involved in preeclampsia. Common cellular functions studied include adhesion, apoptosis, proliferation, migration, and invasion. At present, most researchers will use endpoint experimental assays that only allow the determination of cell function at a single time point, with the need to repeat the experiment for an alternate time point. Here, we describe an electrical impedance-based tool that allows real-time monitoring of cells, which enables the efficient assessment of multiple time points over the duration of a single experiment.
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http://dx.doi.org/10.1007/978-1-4939-7498-6_20 | DOI Listing |
Soft Robot
December 2024
Department of Mechanical Engineering, Yonsei University, Seoul, Republic of Korea.
Wearable robots, especially those composed of soft materials, are increasingly attracting interest due to their comfort, ease of donning and doffing, and their ability to provide assistance across various applications. In wearable robotics, striking a balance between ensuring low impedance for wearer comfort and providing sufficient assistive force is a notable design challenge. In this study, we propose exploiting impedance variation in accordance with the types of muscle contraction in the human body.
View Article and Find Full Text PDFCells
October 2024
Walther Straub Institute for Pharmacology and Toxicology, Member of the German Center for Lung Research (DZL), Medical Faculty, LMU-Munich, Nussbaumstrasse 26, 80336 Munich, Germany.
Transient receptor potential vanilloid 4 (TRPV4) channels have been associated with numerous pulmonary pathologies, including hypertension, asthma, and acute lung injury. However, their role in the alveolar epithelium remains unclear. We performed impedance-based resistance measurements in primary differentiated alveolar epithelial type I (AT1) cells from wild-type (WT) and TRPV4-deficient (TRPV4-/-) C57/BL6J mice to detect changes in AT1 barrier integrity upon TRPV4 activation.
View Article and Find Full Text PDFBiosensors (Basel)
September 2024
Department of Electrical and Computer Engineering, Rutgers University, Piscataway, NJ 08854, USA.
Nanowell-based impedance-based label-free biosensors have demonstrated significant advantages in sensitivity, simplicity, and accuracy for detecting cancer biomarkers and macromolecules compared to conventional impedance-based biosensors. Although nanowell arrays have previously been employed for biomarker detection, a notable limitation exists in the photolithography step of their fabrication process, leading to a reduced efficiency rate. Historically, the diameter of these nanowells has been 2 μm.
View Article and Find Full Text PDFBiosensors (Basel)
August 2024
Nanobioelectronics Laboratory (NBEL), Department of Biomedical Engineering, Faculty of Engineering Sciences, Ilse Katz Institute for Nanoscale Science and Technology, Zelman Center for Brain Science Research, Ben-Gurion University of the Negev, Building 64, Rm 204, Beer Sheva 8410501, Israel.
Microfluidics have revolutionized cell culture by allowing for precise physical and chemical environmental control. Coupled with electrodes, microfluidic cell culture can be activated or have its changes sensed in real-time. We used our previously developed reliable and stable microfluidic device for cell growth and monitoring to design, fabricate, and characterize a whole-channel impedance-based sensor and used it to systematically assess the electrical and electrochemical influences of microfluidic channel boundaries coupled with varying electrode sizes, distances, coatings, and cell coverage.
View Article and Find Full Text PDFBMC Ophthalmol
August 2024
Biophysics and Cellular Biotechnology Department, Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 8 Eroii Sanitari blvd., Bucharest, 050474, Romania.
Background: Age-related macular degeneration (AMD) is a prevalent ocular pathology affecting mostly the elderly population. AMD is characterized by a progressive retinal pigment epithelial (RPE) cell degeneration, mainly caused by an impaired antioxidative defense. One of the AMD therapeutic procedures involves injecting healthy RPE cells into the subretinal space, necessitating pure, healthy RPE cell suspensions.
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