AI Article Synopsis
- A quantitative calibration method for optical tweezers is essential for measuring forces in complex viscoelastic environments like living cell cytoplasm.
- An improved version of the fluctuation-dissipation-theorem method allows for in situ calibration without needing prior knowledge of the trapped object.
- The method successfully extracted viscoelastic properties and force constants by calibrating an optical trap in various media, including water and solutions, and inside living fission yeast (S. pombe).
Article Abstract
In order to apply optical tweezers-based force measurements within an uncharacterized viscoelastic medium such as the cytoplasm of a living cell, a quantitative calibration method that may be applied in this complex environment is needed. We describe an improved version of the fluctuation-dissipation-theorem calibration method, which has been developed to perform in situ calibration in viscoelastic media without prior knowledge of the trapped object. Using this calibration procedure, it is possible to extract values of the medium's viscoelastic moduli as well as the force constant describing the optical trap. To demonstrate our method, we calibrate an optical trap in water, in polyethylene oxide solutions of different concentrations, and inside living fission yeast (S. pombe).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6910605 | PMC |
| http://dx.doi.org/10.1063/1.5012782 | DOI Listing |
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