AI Article Synopsis
- A high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method was developed for measuring amlodipine levels in dried blood spot samples.
- Chromatographic separation utilized a Waters XBridge C18 column with a specific gradient elution of water and acetonitrile mixed with formic acid.
- The method was validated according to FDA guidelines and was clinically applied in a large study to measure blood concentrations of amlodipine in patients.
Article Abstract
A sensitive and specific method, utilising high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) was developed for the quantitative determination of amlodipine in dried blood spot (DBS) samples. Chromatographic separation was achieved using a Waters XBridge C18 column with gradient elution of a mixture of water and acetonitrile containing 0.1% formic acid (v/v). Amlodipine was quantified using a Waters Quattro Premier mass spectrometer coupled with an electro-spray ionization (ESI) source in positive ion mode. The MRM transitions of 408.9 m/z→238.1m/z and 408.9→294.0 m/z were used to quantify and qualify amlodipine, respectively. The method was validated across the concentration range of 0.5-30ng/mL by assessing specificity, sensitivity, linearity, precision, accuracy, recovery and matrix effect according to the Food and Drug Administration (FDA) guidelines. This method was also validated clinically within a large pharmacoepidemiological study in which amlodipine blood concentration was determined in patients who had been prescribed this medication.
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| http://dx.doi.org/10.1016/j.jchromb.2017.11.018 | DOI Listing |
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