Let there be light: Chemiluminescence provides a bright detection signal against a dark background and offers an excellent signal-to-noise ratio for analysis. Now, a chemiluminescent probe for cathepsin B has been developed that provides a 16,000-fold improvement in sensitivity for detecting protease activity.

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.201711228DOI Listing

Publication Analysis

Top Keywords

ultrasensitive chemiluminescent
4
chemiluminescent detection
4
detection cathepsin b
4
cathepsin b insights
4
insights frontier
4
frontier chemiluminescent
4
chemiluminescent imaging
4
imaging light
4
light chemiluminescence
4
chemiluminescence bright
4

Similar Publications

Electropositive Magnetic Fluorescent Nanoprobe-Mediated Immunochromatographic Assay for the Ultrasensitive and Simultaneous Detection of Bacteria.

Adv Sci (Weinh)

January 2025

Department of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510000, China.

Immunochromatographic assays (ICAs) provide simple and rapid strategies for bacterial diagnosis but still suffer from the problems of low sensitivity and high dependency on paired antibodies. Herein, the broad-spectrum capture and detection capability of the antibody-free electropositive nanoprobe are clarified for bacteria for the first time and an ultrasensitive fluorescent ICA platform is constructed for the simultaneous diagnosis of multiple pathogens. A magnetic multilayer quantum dot nanocomposite with an amino-embedded SiO shell (MagMQD@Si) is designed to enrich bacteria from solutions effectively, offer high luminescence, and reduce background signals on test strips, thus greatly improving the sensitivity and stability of ICA technique for pathogen.

View Article and Find Full Text PDF

The anti-Stokes emission of photon upconversion nanoparticles (UCNPs) facilitates their use as labels for ultrasensitive detection in biological samples as infrared excitation does not induce autofluorescence at visible wavelengths. The detection of extremely low-abundance analytes, however, remains challenging as it is impossible to completely avoid nonspecific binding of label conjugates. To overcome this limitation, we developed a novel hybridization complex transfer technique using UCNP labels to detect short nucleic acids directly without target amplification.

View Article and Find Full Text PDF

Afterglow luminescence provides ultrasensitive optical detection by minimizing tissue autofluorescence and increasing the signal-to-noise ratio. However, due to the lack of suitable unimolecular afterglow scaffolds, current afterglow agents are nanocomposites containing multiple components with limited afterglow performance and have rarely been applied for cancer theranostics. Herein, we report the synthesis of a series of oxathiine-containing donor-acceptor block semiconducting polymers (PDCDs) and the observation of their high photoreactivity and strong near-infrared (NIR) afterglow luminescence.

View Article and Find Full Text PDF

Ultrasensitive platform for the determination of biothiols using aggregation-induced emission of gold-cysteine nanosheets.

Biosens Bioelectron

January 2025

Guangdong Laboratory of Artificial Intelligence and Digital Economy (SZ), Shenzhen University, Shenzhen, 518060, China; Marshall Laboratory of Biomedical Engineering, Shenzhen Key Laboratory of Nano-Biosensing Technology, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen University, Shenzhen, 518060, China. Electronic address:

Highly ordered ultrathin nanosheets (NSs) of Au(I)-Cys were fabricated through aggregation-induced supramolecular self-assembly triggered by an extended agitation in an alkaline environment. The synthesized Au(I)-Cys NSs exhibited intense luminescence and exceptional chirality. Remarkably, additions of biothiols to Au(I)-Cys NSs have significantly enhanced their luminescence emission, and circular dichroism properties coupled with morphological modulations into nanoflowers, nanodendrites, or closely packed aggregates.

View Article and Find Full Text PDF

This study introduces a novel synchronous spectrofluorimetry coupled with chemometric tools for the determination of tenofovir and dolutegravir antiretroviral drugs. Utilizing partial least squares regression (PLS) fine-tuned by genetic algorithm as variable selection tool, the developed models demonstrate greater sensitivity, cost-effectiveness, and reduced environmental impact compared to traditional HPLC methods. The model's validation was further confirmed using external validation in addition to QC samples as per ICH M10 guidelines, which yielded high accuracy ranged between 94.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!