Background: This study takes a whole-transcriptome approach to assess gene expression changes in response to glyphosate treatment in glyphosate-resistant fleabane. We assessed gene expression changes in both susceptible and resistant lines so that the glyphosate death response could be quantified, and constitutively expressed candidate resistance genes identified. There are three copies of the glyphosate target site (5-enolpyruvylshikimate-3-phosphate; EPSPS) gene in Conyza and because Conyza bonariensis is allohexaploid, there is a baseline nine copies of the gene in any individual.
Results: Many genes were differentially expressed in response to glyphosate treatment. Known resistance mutations are present in EPSPS2 but they are present in a glyphosate-susceptible line as well as resistant lines and therefore not sufficient to confer resistance. EPSPS1 is expressed four times more than EPSPS2, further reducing the overall contribution of these mutations.
Conclusion: We demonstrate that glyphosate resistance in C. bonariensis is not the result of EPSPS mutations or overexpression, but due to a non-target-site mechanism. A large number of genes are affected by glyphosate treatment. We present a list of candidate non-target-site-resistance (NTSR) genes in fleabane for future studies into these mechanisms. © 2017 Society of Chemical Industry.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/ps.4804 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Charting water quality improvements and practice reversion with pesticide interventions at catchment scale.
Sci Total Environ
January 2025
Agri-Environment Branch, Agri-Food and Bioscience Institute (AFBI), Belfast, UK.
Freshwater quality, and the impacts of farming practice on drinking water supplies, are of concern in many countries and time-limited catchment management interventions are commonly used to improve water quality. However, ending such schemes may result in practice reversion. This study adopts an interdisciplinary approach combining evidence from water quality monitoring data with a behavioural study of farmers to explore changes in land use practice with reference to the pesticide MCPA (2-methyl-4-chlorophenoxyacetic acid) following a catchment-based management scheme delivered in the cross-border Derg catchment in Northern Ireland/Ireland between 2018 and 2021.
View Article and Find Full Text PDFBromelia pinguin Extract Mitigates Glyphosate-Induced Toxicity in Human Cells.
Cureus
November 2024
Genotoxicology Laboratory, Universidad Autónoma de Occidente, Los Mochis, MEX.
Introduction: Extensive agricultural activity results in significant exposure to pesticides, particularly glyphosate, which has been linked to immunological disorders, including apoptosis and inflammation. , a species from the Bromeliaceaefamily native to Mexico, is traditionally used in folk medicine for its medicinal properties, including anti-inflammatory effects. This research aimed to evaluate the protective effects of extract on human peripheral blood mononuclear cells (PBMCs) exposed to Faena®, a commercially available glyphosate-based herbicide.
View Article and Find Full Text PDFRevealing novel protein interaction partners of glyphosate in Escherichia coli.
Environ Int
December 2024
Department of Molecular Toxicology, Helmholtz-Centre for Environmental Research GmbH (UFZ), Leipzig, Germany.
Despite all debates about its safe use, glyphosate remains the most widely applied active ingredient in herbicide products, with renewed approval in the European Union until 2033. Non-target organisms are commonly exposed to glyphosate as a matter of its mode of application, with its broader environmental and biological impacts remaining under investigation. Glyphosate displays structural similarity to phosphoenolpyruvate (PEP), thereby competitively inhibiting the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), crucial for the synthesis of aromatic amino acids in plants, fungi, bacteria, and archaea.
View Article and Find Full Text PDFMorphological and cellular effects in Boana faber tadpoles (Anura: Hylidae) exposed to atrazine-based herbicide and glyphosate-based herbicide and their mixtures.
Environ Sci Pollut Res Int
December 2024
Laboratório de Ecologia e Conservação, Universidade Federal da Fronteira Sul, Campus Erechim, RS 135 - km 72, nº 200, Erechim, RS, Brazil.
Atrazine and glyphosate are considered some of the main pollutants for aquatic ecosystems, directly and indirectly affecting non-target organisms, such as amphibians. This study aimed to evaluate the sublethal effects of different concentrations of atrazine-based herbicide (ABH) and glyphosate-based herbicide (GBH) commercial formulations, both individually and in a mixture, through toxicity tests on the larval stage of Boana faber. Tadpoles were exposed to concentrations of ABH (2, 9.
View Article and Find Full Text PDFSusceptibility of solitary bees to agrochemicals highlights gaps in bee risk assessment.
Environ Toxicol Pharmacol
January 2025
Dipartimento di Agricoltura, Alimentazione e Ambiente, sez. Entomologia applicata. Università degli Studi di Catania, Via S. Sofia 100, Catania 95123, Italy.
Ground-nesting solitary bees are the most abundant bee species in the xeric areas of the world, but the effects of agrochemicals on them have been little studied. Herein, we evaluated the topical toxicity of an insecticide, a herbicide, and an essential oil on Mediterranean ground-nesting bees (Andrena impunctata, A. nigroolivacea, A.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!