Mig-6 deficiency cooperates with oncogenic Kras to promote mouse lung tumorigenesis.

Objectives: Lung cancer is the leading cause of cancer related deaths worldwide and mutation activating KRAS is one of the most frequent mutations found in lung adenocarcinoma. Identifying regulators of KRAS may aid in the development of therapies to treat this disease. The mitogen-induced gene 6, MIG-6, is a small adaptor protein modulating signaling in cells to regulate the growth and differentiation in multiple tissues. Here, we investigated the role of Mig-6 in regulating adenocarcinoma progression in the lungs of genetically engineered mice with activation of Kras.

Materials And Methods: Using the CCSP mouse to specifically activate expression of the oncogenic Kras in Club cells, we investigated the expression of Mig-6 in CCSPKras-induced lung tumors. To determine the role of Mig-6 in Kras-induced lung tumorigenesis, Mig-6 was conditionally ablated in the Club cells by breeding Mig6 mice to CCSPKras mice, yielding CCSPMig-6Kras mice (Mig-6Kras).

Results: We found that Mig-6 expression is decreased in CCSPKras-induced lung tumors. Ablation of Mig-6 in the Kras background led to enhanced tumorigenesis and reduced life expectancy. During tumor progression, there was increased airway hyperplasia, a heightened inflammatory response, reduced apoptosis in Kras mouse lungs, and an increase of total and phosphorylated ERBB4 protein levels. Mechanistically, Mig-6 deficiency attenuates the cell apoptosis of lung tumor expressing KRAS partially through activating the ErbB4 pathway.

Conclusions: In summary, Mig-6 deficiency promotes the development of Kras-induced lung adenoma through reducing the cell apoptosis in Kras mouse lungs partially by activating the ErbB4 pathway.