Background: A few methanogens have been previously recovered from periodontitis lesions, yet their repertoire may not be completed. We recovered a previously unreported methanogen species in this situation.
Case Presentation: A 64-year-old Caucasian woman was diagnosed with chronic, severe generalized periodontitis. In the presence of negative controls, an 18-month culture of periodontal pockets in anaerobe Hungate tube yielded "Methanobrevibacter massiliense" and Pyramidobacter piscolens.
Conclusions: This case report provides evidence of the symbiotic strategy deployed by the methanogens and the anaerobes, and reports the first culture of a new methanogen, "M. massiliense".
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http://dx.doi.org/10.1186/s13104-017-2980-3 | DOI Listing |
Microorganisms
January 2024
IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Aix-Marseille University, 13005 Marseille, France.
Among oral microbiota methanogens, () has remained less studied than the well-characterised and cultivated methanogens and . has been associated with different oral pathologies and was co-isolated with the bacterium () in one case of severe periodontitis. Here, reporting on two additional necrotic pulp cases yielded the opportunity to characterise two co-cultivated isolates, both with , as non-motile, 1-2-µm-long and 0.
View Article and Find Full Text PDFBMC Oral Health
October 2019
Aix Marseille University, IRD, MEPHI, IHU-Méditerranée Infection, 19-21, Boulevard Jean Moulin, 13005, Marseille, France.
Background: The oral cavity of humans is inhabited by several hundreds of bacterial species and other microorganisms such as fungi and archaeal methanogens. Regarding methanogens, data have been obtained from oral cavity samples collected in Europe, America and Asia. There is no study published on the presence of methanogens in the oral cavity in persons living in Africa.
View Article and Find Full Text PDFFront Public Health
March 2019
Aix Marseille University, IRD, MEPHI, IHU-Méditerranée Infection, Marseille, France.
The authors report the cases of 9 patients eventually diagnosed with methanogenic archaea refractory or recalcitrant chronic rhinosinusitis, a condition known to involve various anaerobic bacteria but in which the role of methanogenic archaea is unknown. The authors retrospectively searched these microorganisms by PCR in surgically-collected sinusal pus specimens from patients diagnosed with refractory sinusitis, defined by the persistance of sinus inflammation and related-symptoms for more than 12 weeks despite appropriate treatment. Of the 116 tested sinus surgical specimens, 12 (10.
View Article and Find Full Text PDFSci Rep
June 2018
Aix-Marseille Univ, IRD, MEPHI, IHU Méditerranée-Infection, Marseille, France.
Methanogens have already been described in periodontitis but not in peri-implantitis. Thirty peri-implantitis samples and 28 control samples were collected in 28 consenting peri-implantitis patients. PCR-sequencing of the 16S rRNA gene was used as a broad-spectrum screening method and results were further confirmed by real-time quantitative PCR targeting the mcrA genes.
View Article and Find Full Text PDFBMC Res Notes
December 2017
UFR Odontologie, Aix-Marseille Université, 27, Boulevard Jean Moulin, Marseille Cedex 5, France.
Background: A few methanogens have been previously recovered from periodontitis lesions, yet their repertoire may not be completed. We recovered a previously unreported methanogen species in this situation.
Case Presentation: A 64-year-old Caucasian woman was diagnosed with chronic, severe generalized periodontitis.
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