AI Article Synopsis
- This protocol outlines a detailed method for analyzing sphingolipids in aqueous humor using shotgun techniques.
- It explains the Bligh and Dyer method for extracting crude lipids from samples.
- The procedure involves using ESI-MS/MS for identifying and quantifying different sphingolipids, along with data processing using MZmine 2.21.
Article Abstract
This protocol provides a step-by-step guide to shotgun sphingolipid analysis of aqueous humor. We describe the Bligh and Dyer crude lipid extraction method and electrospray ionization tandem mass spectrometry (ESI-MS/MS) coupled with MZmine 2.21 data processing for identification and ratiometric quantitation of sphingosine, sphingosine-1-phosphate, sphingomyelin, and ceramide.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545364 | PMC |
| http://dx.doi.org/10.1007/978-1-4939-7407-8_10 | DOI Listing |
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