AI Article Synopsis
- iTRAQ 4plex method allows the simultaneous analysis of up to four samples, enhancing sensitivity and the depth of proteomic studies.
- The process involves labeling human aqueous humor samples, followed by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for peptide identification and quantitation.
- This protocol is adaptable for other animal specimens but may require pooling samples to ensure enough protein is available for labeling.
Article Abstract
iTRAQ 4plex method enables multiplexing of up to four samples for simultaneous quantitation to improve sensitivity and scope of proteomic analysis. Here, we describe iTRAQ 4plex labeling of human aqueous humor specimens followed by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and data analysis for peptide identification and quantitation using Proteome Discoverer software. The protocol can be applied for other animals as well; however, pooling of specimens may be required to obtain sufficient amount of protein for labeling.
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| http://dx.doi.org/10.1007/978-1-4939-7407-8_9 | DOI Listing |
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