In this article, we report original data on the designation of the primers for full-length cDNA amplification and the internal cDNA amplification of red color-related pigment-binding protein derived from shrimp shell. Data on the color shifts of different soluble proteins under 100 °C 10 min heat treatment and the effects of heating temperatures (from 30 to 100 °C) on the color changes of crude water-soluble proteins are also included in this report. For further details and experimental findings please refer to the article "Isolation and cDNA cloning of a novel red color-related pigment-binding protein derived from the shell of shrimp, " (Chuang et al., 2017) [1].
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http://dx.doi.org/10.1016/j.dib.2017.11.025 | DOI Listing |
Talanta
December 2024
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, PR China. Electronic address:
Staphylococcus aureus (S. aureus) has been identified as a indicator of food contamination. In this study, a sensitive and accurate biosensor strategy for S.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
Department of Anatomy & Embryology, Leiden University Medical Center, Leiden, The Netherlands.
ScarTrace is a CRISPR/Cas9-based genetic lineage tracing method that allows for uniquely barcoding the DNA of single cells at a target GFP sequence during developing zebrafish embryos. Single cells from barcoded adult zebrafish can be isolated from various tissues (e.g.
View Article and Find Full Text PDFFood Chem
December 2024
School of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
The conductivity of Zn-MOF-on-Co-MOF synthesized by one-pot method is improved by searching for the optimum carbonization temperature, which overcomes the limitation of traditional MOF. In order to further enhance electron transfer, the mesoporous PtPdCo trimetal was introduced, which provided considerable load capacity for methylene blue (MB) and reverse complementary DNA (sDNA), and also showed excellent catalytic activity for MB. In this study, the conductivity of aptasensor was improved by modifying carbonized MOF as the base material.
View Article and Find Full Text PDFFront Immunol
January 2025
Aix Marseille Université, CNRS, INSERM, Centre d'Immunologie de Marseille-Luminy, Marseille, France.
Parallel analysis of phenotype, transcriptome and antigen receptor sequence in single B cells is a useful method for tracking B cell activation and maturation during immune responses. However, in most cases, the specificity and affinity of the B cell antigen receptor cannot be inferred from its sequence. Antibody cloning and expression from single B cells is then required for functional assays.
View Article and Find Full Text PDFFront Endocrinol (Lausanne)
December 2024
Rare Disease Research Group, Molecular (Epi) Genetics Laboratory, Bioaraba Health Research Institute, Araba University Hospital, Vitoria-Gasteiz, Spain.
Objective: To identify the genetic cause underlying the methylation defect in a patient with clinical suspicion of PHP1B/iPPSD3.
Design: Imprinting is an epigenetic mechanism that allows the regulation of gene expression. The locus is one of the loci within the genome that is imprinted.
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