AI Article Synopsis
- Optical clearing is gaining traction in neuroscience, but traditional corrosive solutions can damage optical components.
- The use of a corrected multimode fibre (MMF) provides a safer, flexible alternative that can operate in corrosive environments without being limited by refractive index issues.
- The study successfully demonstrates fluorescence and Raman imaging capabilities using MMFs with cleared mouse brain tissue, highlighting their versatility for various imaging techniques.
Article Abstract
Optical clearing is emerging as a popular approach particularly for studies in neuroscience. However the use of corrosive clearing solutions typically requires sophisticated objectives or extreme care with optical components chosen for single- or multi-photon imaging. In contrast to the use of complex, custom-made microscope objectives, we show that the use of a corrected multimode fibre (MMF) offers a route that is resistant to corrosion, can be used in clearing media, is not constrained by the refractive index of the immersion medium and offers flexible working distances. Using a corrected MMF, we demonstrate fluorescence imaging of beads and stained neuroblastoma cells through optically cleared mouse brain tissue, as well as imaging in an extreme oxidative environment to show the versatility of our approach. Additionally, we perform Raman imaging of polystyrene beads in clearing media to demonstrate that this approach may be used for vibrational spectroscopy of optically cleared samples.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695962 | PMC |
| http://dx.doi.org/10.1364/BOE.8.005179 | DOI Listing |
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