Comparison of four methods of surface roughness assessment of corneal stromal bed after lamellar cutting.

Biomed Opt Express

Corneal Graft Biology, Engineering and Imaging Laboratory, BiiGC, EA2521, Federative Institute of Research in Sciences and Health Engineering, Faculty of Medicine, Jean Monnet University, 10 rue de la Marandière, 42055 Saint-Etienne Cédex 02, France.

Published: November 2017

Corneal lamellar cutting with a blade or femtosecond laser (FSL) is commonly used during refractive surgery and corneal grafts. Surface roughness of the cutting plane influences postoperative visual acuity but is difficult to assess reliably. For the first time, we compared chromatic confocal microscopy (CCM) with scanning electron microscopy, atomic force microscopy (AFM) and focus-variation microscopy (FVM) to characterize surfaces of variable roughness after FSL cutting. The small area allowed by AFM hinders conclusive roughness analysis, especially with irregular cuts. FVM does not always differentiate between smooth and rough surfaces. Finally, CCM allows analysis of large surfaces and differentiates between surface states.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5695945PMC
http://dx.doi.org/10.1364/BOE.8.004974DOI Listing

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