Two major sulfoglycolipids, sulfatide (SO3-3Gal-ceramide) and seminolipid (SO3-3Gal-alkylacylglycerol) exist in mammals. Sulfatide is abundant in the myelin sheath and seminolipid is unique to the spermatogenic cells. The carbohydrate moiety of sulfatide and seminolipid is identical and synthesized by common enzymes: ceramide galactosyltransferase (CGT) and cerebroside sulfotransferase (CST). We have purified CST homogenously, cloned the CST gene and generated CST-knockout mice. CST-null mice completely lack sulfoglycolipids all over the body. Analysis of CST-null mice has revealed that sulfatide is an essential component for the axo-glial junction at the paranode region and regulates terminal differentiation of oligodendrocytes, and that seminolipid is responsible for the formation of a functional lactate transporter assembly to take up the critical energy source for spermatocytes. We have developed a new analytical method termed EMARS to identify co-clustered molecules in the membrane microdomains in order to elucidate the functional molecules that collaborate with sulfoglycolipids.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/jb/mvx078 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Population of CD4CD8 Double-Positive T Cells Associated with Risk of Plasma Leakage in Dengue Viral Infection.
Viruses
January 2022
Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, La Jolla, CA 92037, USA.
According to the WHO 2009 classification, dengue with warning signs is at the risk of developing severe form of dengue disease. One of the most important warning signs is plasma leakage, which can be a serious complication associated with higher morbidity and mortality. We report that the frequency of CD4CD8 double-positive (DP) T cells is significantly increased in patients at risk of developing plasma leakage.
View Article and Find Full Text PDFLipopeptide families at the interface between pathogenic and beneficial -plant interactions.
Crit Rev Microbiol
August 2020
Centre of Microbial and Plant Genetics, Faculty of Bioscience Engineering, KU Leuven, Heverlee-Leuven, Belgium.
Lipopeptides (LPs) are a prominent class of molecules among the steadily growing spectrum of specialized metabolites retrieved from , in particular soil-dwelling and plant-associated isolates. Among the multiple LP families, pioneering research focussed on phytotoxic and antimicrobial cyclic lipopeptides (CLPs) of the ubiquitous plant pathogen (syringomycin and syringopeptin). Their non-ribosomal peptide synthetases (NRPSs) are embedded in biosynthetic gene clusters (BGCs) that are tightly co-clustered on a pathogenicity island.
View Article and Find Full Text PDFBiological functions of sulfoglycolipids and the EMARS method for identification of co-clustered molecules in the membrane microdomains.
J Biochem
April 2018
Department of Biochemistry, Kochi University Medical School, Kohasu, Oko-cho, Nankoku, Kochi 783-8505, Japan.
Two major sulfoglycolipids, sulfatide (SO3-3Gal-ceramide) and seminolipid (SO3-3Gal-alkylacylglycerol) exist in mammals. Sulfatide is abundant in the myelin sheath and seminolipid is unique to the spermatogenic cells. The carbohydrate moiety of sulfatide and seminolipid is identical and synthesized by common enzymes: ceramide galactosyltransferase (CGT) and cerebroside sulfotransferase (CST).
View Article and Find Full Text PDFA systematic analysis reveals heterogeneous changes in the endocytic activities of cancer cells.
Cancer Res
November 2015
Department of Cell Biology, UT Southwestern Medical Center, Dallas, Texas.
Metastasis is a multistep process requiring cancer cell signaling, invasion, migration, survival, and proliferation. These processes require dynamic modulation of cell surface proteins by endocytosis. Given this functional connection, it has been suggested that endocytosis is dysregulated in cancer.
View Article and Find Full Text PDFEach GPI-anchored protein species forms a specific lipid raft depending on its GPI attachment signal.
Glycoconj J
October 2015
Center for Innovate and Translational Medicine, Kochi University Medical School, Nankoku, Kochi, 783-8505, Japan.
We previously reported a method, termed enzyme-mediated activation of radical sources (EMARS) for analysis of co-clustered molecules with horseradish peroxidase (HRP) fusion proteins expressed in living cells. This method is featured by radical formation of labeling reagents by HRP. In the current study, we have employed another labeling reagent, fluorescein-conjugated tyramide (FT) instead of the original arylazide compounds.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!