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Isolation and identification of probiotic from local dairy and evaluating their antagonistic effect on pathogens. | LitMetric
Objective : Probiotics such as lactobacilli prevent the development of a wide range of human and animal's pathogens. The aim of this study was evaluation of antagonistic effect of isolated lactobacilli from local dairy products against three standard strains of , , and .Materials And Methods : Twenty samples of local dairy products including cow milk, buffalo milk, cheese, and yogurt were collected from different areas of Ahwaz city. Antimicrobial disc diffusion method was applied on (ATCC-6538), (ATCC-12711), and (ATCC-27853). Antimicrobial effects of isolates were evaluated by disc diffusion test on Mueller-Hinton agar medium plated with three pathogens.Results : Obtained results showed that only three strains of isolated lactobacilli of local dairy samples had inhibitory effects on understudy pathogens including , , and . All three isolates showed moderate activity (inhibition zone <15 mm) except of and that had relatively strong activity (inhibition zone ≥15 mm) against and , respectively.Conclusion : These bacteria can be raised for the production of various kinds of food, pharmaceutical products, and functional foods.
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