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DNAzyme-mediated isothermal catalytic hairpin assembly for rapid and enzyme-free amplified detection of lead(Ⅱ) ion.
J Hazard Mater
January 2025
Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai Ocean University, Shanghai 201306, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China; Shanghai Ocean University, Shanghai 201306, China. Electronic address:
The detection of heavy metal ions, particularly lead (Pb²⁺), in environmental samples is crucial for public health and safety. Current nucleic acid signal amplification methods for Pb²⁺ detection often rely on biological enzymes and are limited in applicability due to high costs, prolonged detection times, and nonspecific adsorption. In this study, we introduce an enzyme-free, DNAzyme-mediated isothermal catalytic hairpin assembly (DMICHA) assay, which combines a DNAzyme-based Pb²⁺ recognition module with a signal amplification process utilizing isothermal catalytic hairpin assembly (CHA).
View Article and Find Full Text PDFA label-free DNAzyme-Mediated biosensor for fluorescent detection of Lead (II) ion.
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:
Lead ion (Pb) is a common environmental contaminant, extremely toxic, persistent, and easily adsorbed, concentrated, and enriched by agricultural products. Ingestion of this ion can result in health problems for humans, including neurological disorders, heart disease, brain damage, and mental deficiency. In this research, a sensitive fluorescent biosensing method for detecting Pb was developed using DNAzyme as the target recognition element and SYBR Green (SG) fluorescent dye as the signal indicator.
View Article and Find Full Text PDFA simple and enzyme-free method for sensitive p53 analysis based on DNAzyme-mediated signal amplification.
Anal Biochem
February 2025
Department of Gastroenterology, Chongqing People's Hospital, Chongqing, 401147, China. Electronic address:
There is an urgent demand for a simple yet extremely accurate biosensor to analyze tumorigenesis. Herein, we present a novel fluorescent and enzyme-free approach for detecting p53 gene cascading proximity ligation-mediated catalytic hairpin assembly and DNAzyme-assisted signal reaction. When the target p53 gene is present, the interaction between p53 and L1 and L2 chains initiates catalytic hairpin assembly and subsequently exposes DNAzyme in the P3 probe.
View Article and Find Full Text PDFA bifunctional MXene@PtPd NPs cascade DNAzyme-mediated fluorescence/colorimetric dual-mode biosensor for Pb determination.
Food Chem
February 2025
College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou 450001, China.
Pb has numerous sources in cosmetics, industrial pollution and other environments. Therefore, sensitive and accurate detection of Pb content is extremely important in food safety. In this work, bifunctional nanomaterials TiC@PtPd NPs with fluorescence quenching effect and peroxidase activity were prepared by in situ growth of platinum‑palladium nanoparticles (PtPd NPs) on the surface of 2D material TiC.
View Article and Find Full Text PDFDNAzyme-mediated fluorescence signal variation of DNA-Ag nanoclusters and construction of an aptasensor for ATP.
Anal Methods
November 2024
School of Biotechnology and Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China.
DNA-templated silver nanoclusters (DNA-AgNCs) are novel nanomaterials with unique fluorescence characteristics. DNAzyme is a functional oligonucleotide that can catalyze the disruption of nucleic acid substrates. In this research, the effect of DNAzyme digestion on the fluorescence property of DNA-AgNCs was explored for the first time.
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