Effect of conventional and contemporary disinfectant techniques on three peri-implantitis associated microbiotas.

Purpose: To evaluate the antiseptic properties of five different disinfectant techniques on three different peri-implantitis (PI) associated biofilms.

Methods: 90 implant titanium disks, with the same thickness and diameter, were prepared and randomly divided into 18 groups (n = 5) based on the microbiota strains (S. aureus, S. epidermidis and C. albicans) and using the following disinfectant techniques:soft laser therapy, photodynamic therapy (PDT), 0.12% NaOCl, 0.2% chlorhexidine, 3% H2O2, and control groups. After forming a protein layer on disk surfaces, the specimens were exposed to the microbial suspensions. After decontamination according to designated techniques, 2% Trypsin protease was administered to isolate the surviving microorganisms. Muller Hinton agar culture was used for microbiota growth. After 48-hour incubation, the standard colony forming unit (CFU) was assayed and the collected data were analyzed by Kruskal-Wallis and Mann-Whitney tests at a significance level of 0.05.

Results: The highest amount of CFU/ml values was shown by C. albicans, which was subjected to PDT (25.12 ± 30.23). The least disinfecting efficacy on S. epidermidis was demonstrated by the laser group (all P-values ≤ 0.01). Nevertheless, all of the groups exhibited significant differences with the control groups (all P-values < 0.01).

Clinical Significance: None of the studied disinfectant techniques had the highest lethal effects on all of the tested microbiotas. Therefore, a combination of these disinfectant techniques, for instance PDT ⁺ 3% H2O2 or 0.2% chlorhexidine, is recommended.