AI Article Synopsis
- The CRISPR-Cas system is a defense mechanism found in many bacteria and archaea that helps protect against invaders like phages, and it can be used to genetically type bacterial strains based on the number of spacers in their CRISPR arrays.
- In a study involving 36 bacterial isolates from patients in hospitals in Thailand, pathogenic genotypes and CRISPR-like sequences were identified, with a significant number showing the most virulent form associated with a specific protein.
- The research indicates that "CRISPR-virulence typing" is a straightforward and reliable method for tracking bacterial epidemiology, and its effectiveness is comparable to traditional methods like RAPD typing.
Article Abstract
Background: Many bacteria and archaea possess a defense system called clustered regularly interspaced short palindromic repeats (CRISPR) associated proteins (CRISPR-Cas system) against invaders such as phages or plasmids. This system has not been demonstrated in . The numbers of spacer in CRISPR array differ among bacterial strains and can be used as a genetic marker for bacterial typing.
Results: A total of 36 isolates were collected from patients in three hospitals located in the central (PBH) and southern (SKH) regions of Thailand. It is of interest that CRISPR-like sequences of this bacterium were detected in encoded for VacA-like protein C. Virulence genes were investigated and the most pathogenic genotype ( s1m1) was detected in 17 out of 29 (58.6%) isolates from PBH and 5 out of 7 (71.4%) from SKH. gene was identified in each one isolate from PBH and SKH. CRISPR-like sequences and virulence genes of 20 isolates of obtained in this study were analyzed and CRISPR-virulence typing was constructed and compared to profiles obtained by the random amplification of polymorphic DNA (RAPD) technique. The discriminatory power (DI) of CRISPR-virulence typing was not different from RAPD typing.
Conclusion: CRISPR-virulence typing in is easy and reliable for epidemiology and can be used for inter-laboratory interpretation.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693588 | PMC |
| http://dx.doi.org/10.1186/s13099-017-0215-8 | DOI Listing |
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