AI Article Synopsis
- A novel screen-printed electrochemical sensor was created to detect bacterial signaling molecules, specifically N-acylhomoserine lactones (AHLs), using a low-cost, disposable carbon electrode enhanced with multi-walled carbon nanotubes for better sensitivity.
- The sensor incorporates rat basophilic leukemia mast cells encapsulated in a hydrogel, which respond to the Pseudomonas aeruginosa signaling molecule 3OC-HSL, exhibiting reduced cell viability at increasing concentrations.
- Testing showed the sensor effectively monitors AHL production in spoiled freshwater fish juice, demonstrating its potential for real-time spoilage detection in fish production.
Article Abstract
A novel screen-printed cell-based electrochemical sensor was developed to assess bacterial quorum signaling molecules, N-acylhomoserine lactones (AHLs). Screen-printed carbon electrode (SPCE), which possesses excellent properties such as low-cost, disposable and energy-efficient, was modified with multi-walled carbon nanotubes (MWNTs) to improve electrochemical signals and enhance the sensitivity. Rat basophilic leukemia (RBL-2H3) mast cells encapsulated in alginate/graphene oxide (NaAgl/GO) hydrogel were immobilized on the MWNTs/SPCE to serve as recognition element. Electrochemical impedance spectroscopy (EIS) was employed to record the cell impedance signal as-influenced by Pseudomonas aeruginosa quorum-sensing molecule, N-3-oxododecanoyl homoserine lactone (3OC-HSL). Experimental results show that 3OC-HSL caused a significant decrease in cell viability in a dose dependent manner. The EIS value decreased with concentrations of 3OC-HSL in the range of 0.1-1μM, and the detection limit for 3OC-HSL was calculated to be 0.094μM. These results were confirmed via cell viability, SEM, TEM analysis. Next, the sensor was successfully applied to monitoring the production of AHLs by spoilage bacteria in three different freshwater fish juice samples which efficiently proved the practicability of this cell based method. Therefore, the proposed cell sensor may serve as an innovative and effective approach to the measurement of quorum signaling molecule and thus provides a new avenue for real-time monitoring the spoilage bacteria in freshwater fish production.
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| http://dx.doi.org/10.1016/j.bios.2017.11.040 | DOI Listing |
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