AI Article Synopsis
- Rapid immunoassay methods have been developed to detect diarrhetic shellfish poisoning (DSP) toxins in seawater, essential for protecting public health and the shellfish industry.* ! -
- These assays specifically target okadaic acid (OA) and two related toxins, achieving a detection limit of about 1 ng OA equiv./mL in seawater, and have been tested on samples from Catalan harbors and the Galician Rias.* ! -
- Findings show that OA levels in seawater correlate with Dinophysis cell counts, but with a delay of 1-2 weeks, indicating that these immunoassays could serve as effective, high-throughput monitoring tools for DSP toxins.* !
Article Abstract
Rapid and cost-effective methods to monitor the presence of diarrhetic shellfish poisoning (DSP) toxins in seawater samples in an easy and reliable manner are required to protect human health and avoid economic losses to shellfish industry. Immunoassays for the detection of okadaic acid (OA) and dinophysistoxin-1 and dinophysistoxin-2 are developed by immobilising OA on self-assembled monothiols or dithiols in an ordered and oriented way, providing an effective limit of detection of ∼1 ng OA equiv./mL seawater. The immunoassays are applied to the analysis of the particulate fraction of seawater samples from two Catalan harbours (NW Mediterranean) and samples collected periodically from the Galician Rias (E Atlantic), as well as a reference mussel sample. Results are in agreement with LC-MS/MS and the certified values. OA concentration in seawater correlates with Dinophysis cell abundance, with a 1-2 weeks lag. The immunoassays provide powerful high-throughput analytical methods potentially applicable as alternative monitoring tools.
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| http://dx.doi.org/10.1016/j.marenvres.2017.11.004 | DOI Listing |
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