AI Article Synopsis
- Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, affecting millions globally and posing challenges for research due to limited genome study tools.
- Recent advancements in gene disruption using the CRISPR/Cas9 system have shown promise, though the efficiency of this technique in T. cruzi can still be improved.
- The authors of this study present a new strategy that enhances previous methods, enabling effective gene disruption for broader applications, including the examination of essential genes.
Article Abstract
Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, affects millions of individuals around the world. Although it has been known for more than a century, the study of T. cruzi has been a challenge, particularly due to the scarcity of tools for genome inquiries. Recently, strategies have been described allowing gene disruption in T. cruzi by the CRISPR/Cas9 nuclease system. Although these strategies demonstrated success in deleting some genes, several aspects could be improved to increase the efficiency of the CRISPR/Cas9 system in T. cruzi. Here, we report a strategy, based on adaptations and improvements of the two previously described systems, that results in efficient gene disruption that can be applied to any target, including the study of essential genes.
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| http://dx.doi.org/10.1016/j.actatropica.2017.11.013 | DOI Listing |
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