A new water soluble and fluorogenic probe (L) that can demonstrate a specific ratiometric detection of a SO derivative (SO) in 100% aqueous medium and live cells has been designed and synthesized. The detection process can be visualized by the naked eye, as the orange-red fluorescence of L turns into a strong blue fluorescence upon interaction with SO. L displayed several beneficial attributes such as detection in complete aqueous medium, extremely fast response time along with high selectivity and sensitivity. The ratiometric sensing was attributed to the selective nucleophilic addition reaction of SO with L. The probe was further used to develop a low cost microfluidic sensor device (μPAD). The probe was biocompatible and its potential to sense SO in mitochondria was captured in live HeLa cells.
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